Induction of Psammosilene tunicoides Adventitious Roots and the Accumulation of Triterpenoid Saponins as Affected by Culture Conditions

In this study, callus of Psammosilene tunicoides was used to induce adventitious roots. It was found that the medium with indole-3-butyric acid (IBA) was most suitable for induction of P. tunicoides adventitious roots from callus. To investigate the effects of concentration and type of auxins on adventitious roots induction, an orthogonal test was deployed. It was shown that 2,4-Dichlorophenoxyacetic acid (2,4-D) 0.3 mg/L+1-Naphthaleneacetic acid (NAA) 0.5 mg/L+ IBA 0.4 mg/L was the favorable auxins combination. We also established a suspension culture system in 500 mL flasks of P. tunicoides adventitious roots, and produced optimal culture conditions for metabolites accumulation with the sucrose concentration of 30 g/L, photoperiod at 10 h/d, and initial pH at 5.8, and IBA concentration at 0.4 mg/L. The resulting analysis highlights the results' useful in facilitating P. tunicoides rich in triterpenoid saponons for large scale cultivation. (C) 2017 Friends Science Publishers