Combined synergetic potential of metformin loaded pectin-chitosan biohybrids nanoparticle for NIDDM

Rationale: Combined therapy is a promising approach over its preference to minimize the dose, adverse effects and enhanced therapeutic efficiency in a various diseases including diabetes. Aim: The present research work is to explore combined synergistic anti-diabetic potential of chitosan and pectin with Metformin (CPM) nano-formulation, with special emphasis on effect of Metformin when integrated with bio polymers. Methods: The biohybrid nanoparticles (CPMNP) were formulated by ionic gelation process. The optimized formulation was examined for various in vitro characterizations, in vivo anti-diabetic potential, biodistribution and targeting efficiency. Results: The optimized biohybrid showed higher content of Metformin 92.1 +/- 3.3% and extended release. The pectin coated nanoparticles had smooth spherical morphology with 581.8 nm size and positive surface charge (+41.76 mV). The biohybrid regulated blood glucose, improved the glucose utilization in vital organs, control the dyslipdimea and renal impairment in diabetic rats. CPMNP-4 significantly enhanced the up regulation of IRA, GLUT-2 and GK receptor gene expression and down regulate the TNF-alpha and IL-6 in pancreas. Also, nanoparticles showed healthier biodistribution simultaneously capability to penetrate in vital organs. Conclusion: The combined synergistic effects of Metformin and biopolymers are due their corresponding mechanism to enhance glucose uptake, minimized the adverse effects during diabetic therapy. (C) 2018 Elsevier B.V. All rights reserved.