Molecular characterisation of Spanish Pseudomonas syringae pv. phaseolicola isolates

被引：0
|
作者：
Oguiza, JA [1 ]
Rico, A [1 ]
Sutra, L [1 ]
Vivian, A [1 ]
Murillo, J [1 ]
机构：
[1] Univ Publ Navarra, Dept Prod Agraria, Pamplona 31006, Spain
来源：
PSEUDOMONAS SYRINGAE AND RELATED PATHOGENS: BIOLOGY AND GENETICS | 2003年
关键词：
genomic groups; rep-PCR; phaseolotoxin; heteroduplex mobility assay;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this work we focused on the molecular characterisation of Spanish Pseudomonas syringae pv. phaseolicola isolates. PCR amplification profiles of repetitive DNA were similar among the Spanish P. s. pv. phaseolicola isolates and the reference P s. pv. phaseolicola strains, but partially different from the patterns shown by P s. pv. glycinea. Taking these profiles into consideration, two P s. pv. phaseolicola genomic groups have been distinguished. Additionally, using a DNA heteroduplex mobility assay (HMA) performed on the internal transcribed spacer (ITS) region between 16S and 23S rRNA genes, it was possible to differentiate Group 1 isolates, which clearly present a unique HMA band, from Group 2 isolates, which show multiple HMA bands.
引用
收藏
页码：697 / 703
页数：7
相关论文
共 50 条
  • [1] Limitations of the current methods for the detection of Spanish strains of Pseudomonas syringae pv. phaseolicola
    Rico, A
    Lopez, R
    Aizpun, MT
    Asensio, C
    Murillo, J
    PSEUDOMONAS SYRINGAE AND RELATED PATHOGENS: BIOLOGY AND GENETICS, 2003, : 545 - 552
  • [2] Remodeling of Lipid A in Pseudomonas syringae pv. phaseolicola In Vitro
    Gerster, Tim
    Wroebel, Michelle
    Hofstaedter, Casey E.
    Schwudke, Dominik
    Ernst, Robert K.
    Ranf, Stefanie
    Gisch, Nicolas
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (04)
  • [3] Milk-Tween agar, a semiselective medium for isolation and differentiation of Pseudomonas syringae pv. syringae, Pseudomonas syringae pv. phaseolicola and Xanthomonas axonopodis pv. phaseoli
    Goszczynska, T
    Serfontein, JJ
    JOURNAL OF MICROBIOLOGICAL METHODS, 1998, 32 (01) : 65 - 72
  • [4] Phenotypic and genetic diversity among Pseudomonas syringae pv. phaseolicola
    Güven, K
    Jones, JB
    Momol, MT
    Dickstein, ER
    JOURNAL OF PHYTOPATHOLOGY, 2004, 152 (11-12) : 658 - 666
  • [5] Multilocus enzyme electrophoresis analysis of Pseudomonas syringae pv. phaseolicola
    Güven, K
    FOLIA MICROBIOLOGICA, 2001, 46 (05) : 371 - 375
  • [6] Pseudomonas syringae pv. phaseolicola: from 'has bean' to supermodel
    Arnold, Dawn L.
    Lovell, Helen C.
    Jackson, Robert W.
    Mansfield, John W.
    MOLECULAR PLANT PATHOLOGY, 2011, 12 (07) : 617 - 627
  • [7] Comparison of ethylene-producing Pseudomonas syringae strains isolated from kudzu (Pueraria lobata) with Pseudomonas syringae pv. phaseolicola and Pseudomonas syringae pv. glycinea
    B. Völksch
    H. Weingart
    European Journal of Plant Pathology, 1997, 103 : 795 - 802
  • [8] Comparison of ethylene-producing Pseudomonas syringae strains isolated from kudzu (Pueraria lobata) with Pseudomonas syringae pv. phaseolicola and Pseudomonas syringae pv. glycinea
    Volksch, B
    Weingart, H
    EUROPEAN JOURNAL OF PLANT PATHOLOGY, 1997, 103 (09) : 795 - 802
  • [9] Molecular Characterization and Taxonomic Assignment of Three Phage Isolates from a Collection Infecting Pseudomonas syringae pv. actinidiae and P. syringae pv. phaseolicola from Northern Italy
    Martino, Gabriele
    Holtappels, Dominique
    Vallino, Marta
    Chiapello, Marco
    Turina, Massimo
    Lavigne, Rob
    Wagemans, Jeroen
    Ciuffo, Marina
    VIRUSES-BASEL, 2021, 13 (10):
  • [10] Molecular characterization of Pseudomonas syringae pv. tomato isolates from Tanzania
    Shenge, K. C.
    Stephan, D.
    Mabagala, R. B.
    Mortensen, C. N.
    Wydra, K.
    PHYTOPARASITICA, 2008, 36 (04) : 338 - 351
12345