Molecular characterisation of Spanish Pseudomonas syringae pv. phaseolicola isolates

被引：0

作者：

Oguiza, JA ^{[1
]}

Rico, A ^{[1
]}

Sutra, L ^{[1
]}

Vivian, A ^{[1
]}

Murillo, J ^{[1
]}

机构：

[1] Univ Publ Navarra, Dept Prod Agraria, Pamplona 31006, Spain

来源：

PSEUDOMONAS SYRINGAE AND RELATED PATHOGENS: BIOLOGY AND GENETICS | 2003年

关键词：

genomic groups; rep-PCR; phaseolotoxin; heteroduplex mobility assay;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work we focused on the molecular characterisation of Spanish Pseudomonas syringae pv. phaseolicola isolates. PCR amplification profiles of repetitive DNA were similar among the Spanish P. s. pv. phaseolicola isolates and the reference P s. pv. phaseolicola strains, but partially different from the patterns shown by P s. pv. glycinea. Taking these profiles into consideration, two P s. pv. phaseolicola genomic groups have been distinguished. Additionally, using a DNA heteroduplex mobility assay (HMA) performed on the internal transcribed spacer (ITS) region between 16S and 23S rRNA genes, it was possible to differentiate Group 1 isolates, which clearly present a unique HMA band, from Group 2 isolates, which show multiple HMA bands.

引用

页码：697 / 703

页数：7

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