Convenient synthesis of nucleoside 5′-triphosphates for RNA transcription

被引：33

作者：

Caton-Williams, Julianne ^{[1
]}

Lin, Lina ^{[1
]}

Smith, Matthew ^{[2
]}

Huang, Zhen ^{[1
,2
]}

机构：

[1] Georgia State Univ, Dept Chem, Atlanta, GA 30303 USA

[2] Georgia State Univ, Dept Biol, Atlanta, GA 30303 USA

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 28期

基金：

美国国家科学基金会;

关键词：

X-RAY CRYSTALLOGRAPHY; ONE-POT SYNTHESIS; ENZYMATIC-SYNTHESIS; TRIPHOSPHATES; 5'-(ALPHA-P-BORANO)TRIPHOSPHATES; 5'-(ALPHA-P-SELENO)TRIPHOSPHATE; NUCLEOTIDES; POLYMERASE; ANALOGS; DNA;

D O I：

10.1039/c1cc12201k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

By generating a selective phosphitylating reagent in situ, nucleoside 5'-triphosphates can be conveniently synthesized in one pot. This novel strategy without nucleoside protection has been developed to largely simplify synthesis of the nucleoside triphosphates. This demonstrated principle can be applied to the 5'-triphosphate synthesis of both native and modified nucleosides.

引用

页码：8142 / 8144

页数：3

共 31 条

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