Immobilization of carbonic anhydrase in alginate and its influence on transformation of CO2 to calcite

被引：65

作者：

Yadav, Raju R. ^{[1
]}

Mudliar, Sandeep N. ^{[2
]}

Shekh, Ajam Y. ^{[1
]}

Fulke, Abhay B. ^{[1
]}

Devi, Sivanesan Saravana ^{[1
]}

Krishnamurthi, Kannan ^{[1
]}

Juwarkar, Asha ^{[3
]}

Chakrabarti, Tapan ^{[1
]}

机构：

[1] NEERI, Div Environm Hlth, Nagpur, Maharashtra, India

[2] NEERI, Environm Biotechnol Div, Nagpur, Maharashtra, India

[3] NEERI, Ecorestorat Div, Nagpur, Maharashtra, India

来源：

PROCESS BIOCHEMISTRY | 2012年 / 47卷 / 04期

关键词：

Immobilization; Carbonic anhydrase; Alginate; Calcite; CO2; sequestration; Immobilized Carbonic anhydrase; DIOXIDE CAPTURE; SEQUESTRATION;

D O I：

10.1016/j.procbio.2011.12.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Present investigation entails carbonic anhydrase (CA) immobilization and its influence on transformation of CO2 to calcite. CA enzyme was immobilized in alginate beads, subsequently maintained its catalytic efficiency after sequential operational cycles. The immobilized beads showed better operational stability by retaining nearly 67% of its initial activity even after six cycles. Batch scale stitches with free and immobilized enzyme revealed that the entrapped CA hydrates CO2 to bicarbonate and/or carbonate which was then made to react with Ca2+ ions to transform into calcite. Calcite was characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The entrapped CA was employed for the performance evaluation with respect to several operational parameters including the influence of enzyme concentration in free and immobilized condition. It was concluded that immobilized CA in alginate beads would have the potential for CO2 sequestration by biomimetic route. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：585 / 590

页数：6

共 24 条

[1] Kinetics of immobilized cyclodextrin gluconotransferase produced by Bacillus macerans ATCC 8244 [J].

Arya, S. K. ;

Srivastava, S. K. .

ENZYME AND MICROBIAL TECHNOLOGY, 2006, 39 (03) :507-510

[2] Solubilization and concentration of carbon dioxide: Novel spray reactors with immobilized carbonic anhydrase [J].

Bhattacharya, S ;

Nayak, A ;

Schiavone, M ;

Bhattacharya, SK .

BIOTECHNOLOGY AND BIOENGINEERING, 2004, 86 (01) :37-46

[3] CO2 hydration by immobilized carbonic anhydrase [J].

Bhattacharya, S ;

Schiavone, M ;

Chakrabarti, S ;

Bhattacharya, SK .

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2003, 38 :111-117

[4] A comparative study of the activity of free and immobilized enzymes and its application to glucose isomerase [J].

CamachoRubio, F ;

JuradoAlameda, E ;

GonzalezTello, P ;

Gonzalez, GL .

CHEMICAL ENGINEERING SCIENCE, 1996, 51 (17) :4159-4165

[5] Environmental applications of immobilized microbial cells: A review [J].

Cassidy, MB ;

Lee, H ;

Trevors, JT .

JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1996, 16 (02) :79-101

[6] Hollow fiber contained hydrogel-CA membrane contactor for carbon dioxide removal from the enclosed spaces [J].

Cheng, Li-Hua ;

Zhang, Lin ;

Chen, Huan-Lin ;

Gao, Cong-Jie .

JOURNAL OF MEMBRANE SCIENCE, 2008, 324 (1-2) :33-43

[7] Immobilization of α-amylase produced by Bacillus circulans GRS 313 [J].

Dey, G ;

Singh, B ;

Banerjee, R .

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, 2003, 46 (02) :167-176

[8] Feasibility study of using brine for carbon dioxide capture and storage from fixed sources [J].

Dziedzic, Daniel ;

Gross, Kenneth B. ;

Gorski, Robert A. ;

Johnson, John T. .

JOURNAL OF THE AIR & WASTE MANAGEMENT ASSOCIATION, 2006, 56 (12) :1631-1641

[9] Optimization of α-amylase immobilization in calcium alginate beads [J].

Ertan, Figen ;

Yagar, Hulya ;

Balkan, Bilal .

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2007, 37 (03) :195-204

[10] Biocatalytic capture of CO2 with carbonic anhydrase and its transformation to solid carbonate [J].

Favre, Nathalie ;

Christ, M. Lorraine ;

Pierre, Alain C. .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2009, 60 (3-4) :163-170

← 1 2 3 →