Posttranslational modifications of apolipoprotein A-II proteoforms in type 2 diabetes

被引：9

作者：

Azizkhanian, Ida ^{[1
]}

Trenchevska, Olgica ^{[2
]}

Bashawri, Yara ^{[1
,3
]}

Hu, Jiaqi ^{[1
]}

Koska, Juraj ^{[4
]}

Reaven, Peter D. ^{[4
]}

Nelson, Randall W. ^{[2
]}

Nedelkov, Dobrin ^{[2
]}

Yassine, Hussein N. ^{[1
]}

机构：

[1] Univ Southern Calif, Dept Med, 2250 Alcazar St,Room 210, Los Angeles, CA 90033 USA

[2] Arizona State Univ, Biodesign, Tempe, AZ USA

[3] King Fahad Med City, Riyadh, Saudi Arabia

[4] Phoenix VA Hlth Care Syst, Dept Med, Phoenix, AZ USA

来源：

JOURNAL OF CLINICAL LIPIDOLOGY | 2016年 / 10卷 / 04期

关键词：

Apolipoprotein A-II; Oxidations; Mass spectrometry; Diabetes; OXIDATIVE STRESS; TRANSGENIC MICE; APOA-II; ATHEROSCLEROSIS; MELLITUS;

D O I：

10.1016/j.jacl.2016.03.001

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

BACKGROUND: Apolipoprotein A-II (apoA-11) is the second most abundant protein in high-density lipoprotein particles. However, it exists in plasma in multiple forms. The effect of diabetes on apoA-II proteoforms is not known. OBJECTIVE: Our objective was to characterize plasma apoA-II proteoforms in participants with and without type 2 diabetes. METHODS: Using a novel mass spectrometric immunoassay, the relative abundance of apoA-II proteoforms was examined in plasma of 30 participants with type 2 diabetes and 25 participants without diabetes. RESULTS: Six apoA-11 proteoforms (monomer, truncated TQ monomer, truncated Q monomer, dimer, truncated Q dimer, and truncated 2Qs dimer) and their oxidized proteoforms were identified The ratios of oxidized monomer and all oxidized proteoforms to the native apoA-11 were significantly greater in the diabetic group (P = .004 and P = .005, respectively) compared with the nondiabetic group. CONCLUSION: The relative abundance of oxidized apoA-II is significantly increased in type 2 diabetes. (C) 2016 National Lipid Association. All rights reserved.

引用

页码：808 / 815

页数：8

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