Neonicotinoid-Coated Zea mays Seeds Indirectly Affect Honeybee Performance and Pathogen Susceptibility in Field Trials

被引：70

作者：

Alburaki, Mohamed ^{[1
,3
]}

Boutin, Sebastien ^{[1
]}

Mercier, Pierre-Luc ^{[1
,3
]}

Loublier, Yves ^{[5
]}

Chagnon, Madeleine ^{[4
]}

Derome, Nicolas ^{[1
,2
]}

机构：

[1] Univ Laval, IBIS, Quebec City, PQ G1K 7P4, Canada

[2] Univ Laval, Dept Biol, Fac Sci & Genie, Quebec City, PQ G1K 7P4, Canada

[3] CRSAD, Quebec City, PQ, Canada

[4] Univ Quebec Montreal, Quebec City, PQ, Canada

[5] CNRS, LEGS, Gif Sur Yvette, France

来源：

PLOS ONE | 2015年 / 10卷 / 05期

关键词：

DEFORMED WING VIRUS; APIS-MELLIFERA; MITOCHONDRIAL-DNA; EXPOSURE; PESTICIDES; INSECTICIDES; POLLINATORS; HYMENOPTERA; BEES; DWV;

D O I：

10.1371/journal.pone.0125790

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Thirty-two honeybee (Apis mellifera) colonies were studied in order to detect and measure potential in vivo effects of neonicotinoid pesticides used in cornfields (Zea mays spp) on honeybee health. Honeybee colonies were randomly split on four different agricultural cornfield areas located near Quebec City, Canada. Two locations contained cornfields treated with a seed-coated systemic neonicotinoid insecticide while the two others were organic cornfields used as control treatments. Hives were extensively monitored for their performance and health traits over a period of two years. Honeybee viruses (brood queen cell virus BQCV, deformed wing virus DWV, and Israeli acute paralysis virus IAPV) and the brain specific expression of a biomarker of host physiological stress, the Acetylcholinesterase gene AChE, were investigated using RT-qPCR. Liquid chromatography-mass spectrometry (LC-MS) was performed to detect pesticide residues in adult bees, honey, pollen, and corn flowers collected from the studied hives in each location. In addition, general hive conditions were assessed by monitoring colony weight and brood development. Neonicotinoids were only identified in corn flowers at low concentrations. However, honeybee colonies located in neonicotinoid treated cornfields expressed significantly higher pathogen infection than those located in untreated cornfields. AChE levels showed elevated levels among honeybees that collected corn pollen from treated fields. Positive correlations were recorded between pathogens and the treated locations. Our data suggests that neonicotinoids indirectly weaken honeybee health by inducing physiological stress and increasing pathogen loads.

引用

页数：20

共 75 条

[61] Comparative analysis of deformed wing virus (DWV) RNA in Apis mellifera and Varroa destructor [J].

Tentcheva, D ;

Gauthier, L ;

Bagny, L ;

Fievet, J ;

Dainat, B ;

Cousserans, F ;

Colin, ME ;

Bergoin, M .

APIDOLOGIE, 2006, 37 (01) :41-50

[62] Neonicotinoids, bee disorders and the sustainability of pollinator services [J].

van der Sluijs, Jeroen P. ;

Simon-Delso, Noa ;

Goulson, Dave ;

Maxim, Laura ;

Bonmatin, Jean-Marc ;

Belzunces, Luc P. .

CURRENT OPINION IN ENVIRONMENTAL SUSTAINABILITY, 2013, 5 (3-4) :293-305

[63] Managed honey bee colony losses in Canada, China, Europe, Israel and Turkey, for the winters of 2008-9 and 2009-10 [J].

van der Zee, Romee ;

Pisa, Lennard ;

Andonov, Sreten ;

Brodschneider, Robert ;

Charriere, Jean-Daniel ;

Chlebo, Robert ;

Coffey, Mary F. ;

Crailsheim, Karl ;

Dahle, Bjorn ;

Gajda, Anna ;

Gray, Alison ;

Drazic, Marica M. ;

Higes, Mariano ;

Kauko, Lassi ;

Kence, Aykut ;

Kence, Meral ;

Kezic, Nicola ;

Kiprijanovska, Hrisula ;

Kralj, Jasna ;

Kristiansen, Preben ;

Martin Hernandez, Raquel ;

Mutinelli, Franco ;

Bach Kim Nguyen ;

Otten, Christoph ;

Ozkirim, Asli ;

Pernal, Stephen F. ;

Peterson, Magnus ;

Ramsay, Gavin ;

Santrac, Violeta ;

Soroker, Victoria ;

Topolska, Grazyna ;

Uzunov, Aleksandar ;

Vejsnaes, Flemming ;

Wei, Shi ;

Wilkins, Selwyn .

JOURNAL OF APICULTURAL RESEARCH, 2012, 51 (01) :91-114

[64] Macro-Invertebrate Decline in Surface Water Polluted with Imidacloprid [J].

Van Dijk, Tessa C. ;

Van Staalduinen, Marja A. ;

Van der Sluijs, Jeroen P. .

PLOS ONE, 2013, 8 (05)

[65] Threats to an ecosystem service: pressures on pollinators [J].

Vanbergen, Adam J. ;

Baude, Mathilde ;

Biesmeijer, Jacobus C. ;

Britton, Nicholas F. ;

Brown, Mark J. F. ;

Brown, Mike ;

Bryden, John ;

Budge, Giles E. ;

Bull, James C. ;

Carvell, Claire ;

Challinor, Andrew J. ;

Connolly, Christopher N. ;

Evans, David J. ;

Feil, Edward J. ;

Garratt, Mike P. ;

Greco, Mark K. ;

Heard, Matthew S. ;

Jansen, Vincent A. A. ;

Keeling, Matt J. ;

Kunis, William E. ;

Marris, Gay C. ;

Memmott, Jane ;

Murray, James T. ;

Nicolson, Susan W. ;

Osborne, Juliet L. ;

Paxton, Robert J. ;

Pirk, Christian W. W. ;

Polce, Chiara ;

Potts, Simon G. ;

Priest, Nicholas K. ;

Raine, Nigel E. ;

Roberts, Stuart ;

Ryabov, Eugene V. ;

Shafir, Sharoni ;

Shirley, Mark D. F. ;

Simpson, Stephen J. ;

Stevenson, Philip C. ;

Stone, Graham N. ;

Termansen, Mette ;

Wright, Geraldine A. .

FRONTIERS IN ECOLOGY AND THE ENVIRONMENT, 2013, 11 (05) :251-259

[66] ALTERATION OF THE HOMING-FLIGHT IN THE HONEY-BEE APIS-MELLIFERA L EXPOSED TO SUBLETHAL DOSE OF DELTAMETHRIN [J].

VANDAME, R ;

MELED, M ;

COLIN, ME ;

BELZUNCES, LP .

ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 1995, 14 (05) :855-860

[67] Exposure to Sublethal Doses of Fipronil and Thiacloprid Highly Increases Mortality of Honeybees Previously Infected by Nosema ceranae [J].

Vidau, Cyril ;

Diogon, Marie ;

Aufauvre, Julie ;

Fontbonne, Regis ;

Vigues, Bernard ;

Brunet, Jean-Luc ;

Texier, Catherine ;

Biron, David G. ;

Blot, Nicolas ;

El Alaoui, Hicham ;

Belzunces, Luc P. ;

Delbac, Frederic .

PLOS ONE, 2011, 6 (06)

[68] CHELEX-100 AS A MEDIUM FOR SIMPLE EXTRACTION OF DNA FOR PCR-BASED TYPING FROM FORENSIC MATERIAL [J].

WALSH, PS ;

METZGER, DA ;

HIGUCHI, R .

BIOTECHNIQUES, 1991, 10 (04) :506-513

[69] Highly sensitive and specific detection of neonicotinoid insecticide imidacloprid in environmental and food samples by a polyclonal antibody-based enzyme-linked immunosorbent assay [J].

Wang, Rongmei ;

Wang, Zhonghui ;

Yang, Hong ;

Wang, Yuzheng ;

Deng, Anping .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2012, 92 (06) :1253-1260

[70] THE GENERALIZATION OF STUDENTS PROBLEM WHEN SEVERAL DIFFERENT POPULATION VARIANCES ARE INVOLVED [J].

WELCH, BL .

BIOMETRIKA, 1947, 34 (1-2) :28-35

← 1 2 3 4 5 6 7 8 →