Human Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering

被引:19
作者
Zhao, Zeqing [1 ]
Sun, Yaxi [1 ]
Qiao, Qingchen [1 ]
Zhang, Li [1 ]
Xie, Xianju [1 ]
Weir, Michael D. [2 ]
Schneider, Abraham [3 ,4 ]
Xu, Hockin H. K. [2 ,4 ,5 ]
Zhang, Ning [1 ]
Zhang, Ke [1 ]
Bai, Yuxing [1 ]
机构
[1] Capital Med Univ, Sch Stomatol, Dept Orthodont, Beijing 100050, Peoples R China
[2] Univ Maryland, Sch Dent, Dept Adv Oral Sci & Therapeut, Biomaterials & Tissue Engn Div, Baltimore, MD 21201 USA
[3] Univ Maryland, Sch Dent, Dept Oncol & Diagnost Sci, Baltimore, MD 21201 USA
[4] Univ Maryland, Sch Med, Marlene & Stewart Greenebaum Canc Ctr, Baltimore, MD 21201 USA
[5] Univ Maryland, Sch Med, Ctr Stem Cell Biol & Regenerat Med, Baltimore, MD 21201 USA
基金
中国国家自然科学基金;
关键词
prevascularization; calcium phosphate scaffold; co-culture; human periodontal ligament stem cells; endothelial cells; bone tissue engineering; CALCIUM-PHOSPHATE SCAFFOLD; BONE; VASCULARIZATION;
D O I
10.3390/ijms222212363
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
(1) Background: Vascularization remains a critical challenge in bone tissue engineering. The objective of this study was to prevascularize calcium phosphate cement (CPC) scaffold by co-culturing human periodontal ligament stem cells (hPDLSCs) and human umbilical vein endothelial cells (hUVECs) for the first time; (2) Methods: hPDLSCs and/or hUVECs were seeded on CPC scaffolds. Three groups were tested: (i) hUVEC group (hUVECs on CPC); (ii) hPDLSC group (hPDLSCs on CPC); (iii) co-culture group (hPDLSCs + hUVECs on CPC). Osteogenic differentiation, bone mineral synthesis, and microcapillary-like structures were evaluated; (3) Results: Angiogenic gene expressions of co-culture group were 6-9 fold those of monoculture. vWF expression of co-culture group was 3 times lower than hUVEC-monoculture group. Osteogenic expressions of co-culture group were 2-3 folds those of the hPDLSC-monoculture group. ALP activity and bone mineral synthesis of co-culture were much higher than hPDLSC-monoculture group. Co-culture group formed capillary-like structures at 14-21 days. Vessel length and junction numbers increased with time; (4) Conclusions: The hUVECs + hPDLSCs co-culture on CPC scaffold achieved excellent osteogenic and angiogenic capability in vitro for the first time, generating prevascularized networks. The hPDLSCs + hUVECs co-culture had much better osteogenesis and angiogenesis than monoculture. CPC scaffolds prevacularized via hPDLSCs + hUVECs are promising for dental, craniofacial, and orthopedic applications.
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页数:15
相关论文
共 37 条
  • [1] Tissue engineering strategies for promoting vascularized bone regeneration
    Almubarak, Sarah
    Nethercott, Hubert
    Freeberg, Marie
    Beaudon, Caroline
    Jha, Amit
    Jackson, Wesley
    Marcucio, Ralph
    Miclau, Theodore
    Healy, Kevin
    Bahney, Chelsea
    [J]. BONE, 2016, 83 : 197 - 209
  • [2] Influence of scaffold morphology on co-cultures of human endothelial and adipose tissue-derived stem cells
    Arnal-Pastor, M.
    Martinez-Ramos, C.
    Valles-Lluch, A.
    Monleon Pradas, M.
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2016, 104 (06) : 1523 - 1533
  • [3] Engineering Pre-vascularized Scaffolds for Bone Regeneration
    Barabaschi, Giada D. G.
    Manoharan, Vijayan
    Li, Qing
    Bertassoni, Luiz E.
    [J]. ENGINEERING MINERALIZED AND LOAD BEARING TISSUES, 2015, 881 : 79 - 94
  • [4] Angiogenic and osteogenic regeneration in rats via calcium phosphate scaffold and endothelial cell co-culture with human bone marrow mesenchymal stem cells (MSCs), human umbilical cord MSCs, human induced pluripotent stem cell-derived MSCs and human embryonic stem cell-derived MSCs
    Chen, Wenchuan
    Liu, Xian
    Chen, Qianmin
    Bao, Chongyun
    Zhao, Liang
    Zhu, Zhimin
    Xu, Hockin H. K.
    [J]. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2018, 12 (01) : 191 - 203
  • [5] von Willebrand factor multimerization and the polarity of secretory pathways in endothelial cells
    da Silva, Mafalda Lopes
    Cutler, Daniel F.
    [J]. BLOOD, 2016, 128 (02) : 277 - 285
  • [6] Fu WL, 2015, TISSUE ENG PT A, V21, P948, DOI [10.1089/ten.tea.2014.0267, 10.1089/ten.TEA.2014.0267]
  • [7] Adult human periodontal ligament-derived stem cells delay retinal degeneration and maintain retinal function in RCS rats
    Huang, Li
    Li, Zongyi
    Tian, Haibin
    Wang, Weiguo
    Cui, Dawei
    Zhou, Zhe
    Chen, Xiao
    Cheung, Herman S.
    Xu, Guo-tong
    Chen, Yu
    [J]. STEM CELL RESEARCH & THERAPY, 2017, 8
  • [8] Human endothelial and foetal femur-derived stem cell co-cultures modulate osteogenesis and angiogenesis
    Inglis, Stefanie
    Christensen, David
    Wilson, David I.
    Kanczler, Janos M.
    Oreffo, Richard O. C.
    [J]. STEM CELL RESEARCH & THERAPY, 2016, 7
  • [9] Angiogenic Effects of Secreted Factors from Periodontal Ligament Stem Cells
    Iwasaki, Kengo
    Akazawa, Keiko
    Nagata, Mizuki
    Komaki, Motohiro
    Peng, Yihao
    Umeda, Makoto
    Watabe, Tetsuro
    Morita, Ikuo
    [J]. DENTISTRY JOURNAL, 2021, 9 (01)
  • [10] Zanthoxylum schinifolium enhances the osteogenic potential of periodontal ligament stem cells
    Kim, So Yeon
    An, Seong Yeong
    Lee, Jeong Seok
    Heo, Jung Sun
    [J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2015, 51 (02) : 165 - 173