Efficient production of male Wolbachia-infected Aedes aegypti mosquitoes enables large-scale suppression of wild populations

被引：245

作者：

Crawford, Jacob E. ^{[1
]}

Clarke, David W. ^{[1
]}

Criswell, Victor ^{[1
]}

Desnoyer, Mark ^{[1
]}

Cornel, Devon ^{[2
]}

Deegan, Brittany ^{[2
]}

Gong, Kyle ^{[1
]}

Hopkins, Kaycie C. ^{[1
]}

Howell, Paul ^{[1
]}

Hyde, Justin S. ^{[1
]}

Livni, Josh ^{[1
]}

Behling, Charlie ^{[1
]}

Benza, Renzo ^{[1
]}

Chen, Willa ^{[1
]}

Dobson, Karen L. ^{[3
]}

Eldershaw, Craig ^{[1
]}

Greeley, Daniel ^{[1
]}

Han, Yi ^{[1
]}

Hughes, Bridgette ^{[1
]}

Kakani, Evdoxia ^{[1
]}

Karbowski, Joe ^{[1
]}

Kitchell, Angus ^{[1
]}

Lee, Erika ^{[1
]}

Lin, Teresa ^{[1
]}

Liu, Jianyi ^{[1
]}

Lozano, Martin ^{[1
]}

MacDonald, Warren ^{[1
]}

Mains, James W. ^{[3
]}

Metlitz, Matty ^{[1
]}

Mitchell, Sara N. ^{[1
]}

Moore, David ^{[1
]}

Ohm, Johanna R. ^{[1
]}

Parkes, Kathleen ^{[1
]}

Porshnikoff, Alexandra ^{[1
]}

Robuck, Chris ^{[1
]}

Sheridan, Martin ^{[1
]}

Sobecki, Robert ^{[1
]}

Smith, Peter ^{[1
]}

Stevenson, Jessica ^{[1
]}

Sullivan, Jordan ^{[1
]}

Wasson, Brian ^{[1
]}

Weakley, Allison M. ^{[1
]}

Wilhelm, Mark ^{[1
]}

Won, Joshua ^{[1
]}

Yasunaga, Ari ^{[1
]}

Chan, William C. ^{[1
]}

Holeman, Jodi ^{[2
]}

Snoad, Nigel ^{[1
]}

Upson, Linus ^{[1
]}

Zha, Tiantian ^{[1
]}

机构：

[1] Verily Life Sci, San Francisco, CA 94080 USA

[2] Consolidated Mosquito Abatement Dist, Parlier, CA USA

[3] MosquitoMate Inc, Lexington, KY USA

[4] Univ Kentucky, Dept Entomol, Lexington, KY USA

来源：

NATURE BIOTECHNOLOGY | 2020年 / 38卷 / 04期

关键词：

ALBOPICTUS DIPTERA-CULICIDAE; CHEMOSTERILIZED MALES; ANOPHELES-ALBIMANUS; EL-SALVADOR; GENETIC-CONTROL; YELLOW-FEVER; RELEASE; ERADICATION; STRAIN; FIELD;

D O I：

10.1038/s41587-020-0471-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The range of the mosquito Aedes aegypti continues to expand, putting more than two billion people at risk of arboviral infection. The sterile insect technique (SIT) has been used to successfully combat agricultural pests at large scale, but not mosquitoes, mainly because of challenges with consistent production and distribution of high-quality male mosquitoes. We describe automated processes to rear and release millions of competitive, sterile male Wolbachia-infected mosquitoes, and use of these males in a large-scale suppression trial in Fresno County, California. In 2018, we released 14.4 million males across three replicate neighborhoods encompassing 293 hectares. At peak mosquito season, the number of female mosquitoes was 95.5% lower (95% CI, 93.6-96.9) in release areas compared to non-release areas, with the most geographically isolated neighborhood reaching a 99% reduction. This work demonstrates the high efficacy of mosquito SIT in an area ninefold larger than in previous similar trials, supporting the potential of this approach in public health and nuisance-mosquito eradication programs. Mosquitoes are nearly eradicated in three suburbs of California using accurately sorted sterile male mosquitoes.

引用

页码：482 / +

页数：13

共 46 条

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