Monoclonal antibodies against Tobacco rattle virus and its application in the detection of TRV isolates

被引：0

作者：

Wu, Xiaoyun ^{[1
,2
]}

Zhou, Xueping ^{[1
]}

Li, Zhengyue ^{[2
]}

Gao, Wenna ^{[3
]}

Wu, Jianxiang ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Biotechnol, Hangzhou 310029, Zhejiang, Peoples R China

[2] Yunnan Agr Univ, Coll Plant Protect, Kunming 650201, Peoples R China

[3] Beijing Entry Exit Inspect & Quarantine Bur, Beijing 100026, Peoples R China

来源：

INDIAN JOURNAL OF VIROLOGY | 2009年 / 20卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Tobacco rattle virus; monoclonal antibody; TAS-ELISA; IC-RT-PCR; PROTEIN; HYBRIDIZATION; TOMATO; ELISA;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two murine hybridoma cell lines secreting monoclonal antibodies (MAbs) against Tobacco rattle virus (TRV) were produced by fusion of mouse myeloma cells (Sp2/0) with spleen cells from BALB/c mice immunized with purified TRV isolate PpK20. Western blot results indicated that both MAbs could react with the coat proteins of four TRV isolates tested. Triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) was optimized with produced MAbs. Also, the sensitivity of TAS-ELISA was compared with that of indirect-ELISA. Finally, immunocapture RT-PCR (IC-RT-PCR) was established for reliable and efficient detection of-my. The two established assays could be used to detect rapidly and reliably TRV in the field plants.

引用

页码：64 / 69

页数：6

共 29 条

[1] Macroarray detection of eleven potato-infecting viruses and Potato spindle tuber viroid [J].

Agindotan, Bright ;

Perry, Keith L. .

PLANT DISEASE, 2008, 92 (05) :730-740

[2] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[3] Genetic variability of genomic RNA 2 of four tobacco rattle tobravirus isolates from potato fields in the Northwestern United States [J].

Crosslin, JM ;

Thomas, PE ;

Hammond, RW .

VIRUS RESEARCH, 2003, 96 (1-2) :99-105

[4]

Crowther J.H., 2001, ELISA GUIDEBOOK, P79

[5] Characterization of tomato zonate spot virus, a new tospovirus in China [J].

Dong, Jia-Hong ;

Cheng, Xiao-Fei ;

Yin, Yue-Yan ;

Fang, Qi ;

Ding, Ming ;

Li, Ting-Ting ;

Zhang, Li-Zhen ;

Su, Xiao-Xia ;

McBeath, Jenifer Huang ;

Zhang, Zhong-Kai .

ARCHIVES OF VIROLOGY, 2008, 153 (05) :855-864

[6]

Harrison B D, 1978, Adv Virus Res, V23, P25, DOI 10.1016/S0065-3527(08)60097-4

[7] COMPARISON OF NUCLEIC-ACID HYBRIDIZATION AND OTHER TESTS FOR DETECTING TOBACCO RATTLE VIRUS IN NARCISSUS PLANTS AND POTATO-TUBERS [J].

HARRISON, BD ;

ROBINSON, DJ ;

MOWAT, WP ;

DUNCAN, GH .

ANNALS OF APPLIED BIOLOGY, 1983, 102 (02) :331-338

[8] Development of recombinant coat protein antibody based IC-RT-PCR for detection and discrimination of sugarcane streak mosaic virus isolates from Southern India [J].

Hema, M ;

Kirthi, N ;

Sreeivasulu, P ;

Savithri, HS .

ARCHIVES OF VIROLOGY, 2003, 148 (06) :1185-1193

[9] SPLENOCYTES OF MICE WITH INDUCED IMMUNOLOGICAL-TOLERANCE TO PLANT ANTIGENS FOR CONSTRUCTION OF HYBRIDOMAS SECRETING TOMATO SPOTTED WILT VIRUS-SPECIFIC ANTIBODIES [J].

HSU, HT ;

WANG, YC ;

LAWSON, RH ;

WANG, M ;

GONSALVES, D .

PHYTOPATHOLOGY, 1990, 80 (02) :158-162

[10] Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and tobacco mosaic tobamoviruses [J].

Jacobi, V ;

Bachand, GD ;

Hamelin, RC ;

Castello, JD .

JOURNAL OF VIROLOGICAL METHODS, 1998, 74 (02) :167-178

← 1 2 3 →