Biohydroxylation reactions catalyzed by enzymes and whole-cell systems

被引：18

作者：

Flitsch, SL ^{[1
]}

Aitken, SJ ^{[1
]}

Chow, CSY ^{[1
]}

Grogan, G ^{[1
]}

Staines, A ^{[1
]}

机构：

[1] Univ Edinburgh, Ctr Prot Technol, Edinburgh, Midlothian, Scotland

来源：

BIOORGANIC CHEMISTRY | 1999年 / 27卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1006/bioo.1999.1135

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biohydroxylation of a number of cyclic substrates (3-24) containing aromatic side chains was used to compare substrate specificity and selectivity of hydroxylation using microbial enzymes and whole-cell biocatalysts. In general, the regioselectivity of reaction was remarkably similar between the different catalysts in that little aromatic or benzylic, but significant aliphatic hydroxylation was observed. However, a more detailed investigation of isolated products showed complementary substrate specificity, functional group compatibility, and regioselectivity of hydroxylation. Substrate specificity and regioselectivity could be further modulated by small changes to the nature of the aromatic side chain, which appears to play an important role in substrate recognition, (C) 1999 Academic Press.

引用

页码：81 / 90

页数：10

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