High-throughput FCS using an LCOS spatial light modulator and an 8 x 1 SPAD array

被引：66

作者：

Colyer, Ryan A. ^{[1
]}

Scalia, Giuseppe ^{[1
]}

Rech, Ivan ^{[2
]}

Gulinatti, Angelo ^{[2
]}

Ghioni, Massimo ^{[2
]}

Cova, Sergio ^{[2
]}

Weiss, Shimon ^{[1
]}

Michalet, Xavier ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90024 USA

[2] Politecn Milan, Dipartimento Elettron & Informaz, I-20133 Milan, Italy

来源：

BIOMEDICAL OPTICS EXPRESS | 2010年 / 1卷 / 05期

关键词：

D O I：

10.1364/BOE.1.001408

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a novel approach to high-throughput Fluorescence Correlation Spectroscopy (FCS) which enables us to obtain one order of magnitude improvement in acquisition time. Our approach utilizes a liquid crystal on silicon spatial light modulator to generate dynamically adjustable focal spots, and uses an eight-pixel monolithic single-photon avalanche photodiode array. We demonstrate the capabilities of this system by showing FCS of Rhodamine 6G under various viscosities, and by showing that, with proper calibration of each detection channel, one order of magnitude improvement in acquisition speed is obtained. More generally, our approach will allow higher throughput single-molecule studies to be performed. (C) 2010 Optical Society of America

引用

页码：1408 / 1431

页数：24

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