Electrochemical Aptamer-Based Sandwich Assays for the Detection of Explosives

被引：105

作者：

Ho, Man Yi ^{[1
]}

D'Souza, Natasha ^{[4
]}

Migliorato, Piero ^{[1
,2
,3
]}

机构：

[1] Univ Cambridge, Elect Engn Div, Cambridge CB3 0FA, England

[2] Kyung Hee Univ, Adv Display Res Ctr, Seoul 130701, South Korea

[3] Kyung Hee Univ, Dept Informat Display, Seoul 130701, South Korea

[4] Def Sci & Technol Lab, Ft Halstead TN14 7BP, Kent, England

来源：

ANALYTICAL CHEMISTRY | 2012年 / 84卷 / 10期

关键词：

2,4,6-TRINITROTOLUENE; TNT; IMMUNOASSAY;

D O I：

10.1021/ac300606n

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Electrochemical impedance spectroscopy (EIS) is used to detect 2,4,6-trinitrotoluene (TNT) in a novel sandwiched structure which relies on the specific interactions between (i) primary amine with TNT and (ii) TNT and anti-TNT aptamer. With pure targets, the assay has a sensitivity of 10(-14) M, a dynamic range of 10(-14)-10(-3) M, and employs a small sample volume (25 mu L). The method's sensitivity is comparable to state of the art optical methods with the added advantages of electrochemical detection, which can be easily miniaturized and implemented into a hand-held device.

引用

页码：4245 / 4247

页数：3

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