Applying DNA barcodes for identification of plant species in the family Araliaceae

被引：69

作者：

Liu, Zhihua ^{[1
]}

Zeng, Xu

Yang, Dan

Chu, Guiyan

Yuan, Zhengrong

Chen, Shilin

机构：

[1] Chinese Acad Med Sci, Inst Med Plant Dev, Ctr Computat Biol & Bioinformat, Beijing 100193, Peoples R China

来源：

GENE | 2012年 / 499卷 / 01期

基金：

中国国家自然科学基金; 北京市自然科学基金;

关键词：

Araliaceae; DNA barcoding; ITS2; SEQUENCES;

D O I：

10.1016/j.gene.2012.02.016

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An effective DNA marker in authentication of the family Araliaceae was screened out of the five DNA regions (matK, rbcL, ITS2, psbA-trnH and ycf5). In the present study, 1113 sequences of 276 species from 23 genera (Araliaceae) were collected from DNA sequencing and GenBank, in which 16 specimens were from 5 provinces in China and Japan. All of the sequences were assessed in the success rates of PCR amplifications, intra- and inter-specific divergence. DNA barcoding gaps and efficiency of identification. Compared with other markers, ITS2 showed superiority in species discrimination with an accurate identification of 85.23% and 97.29% at the species and genus levels, respectively, in plant samples from the 589 sequences derived from Araliaceae. Consequently, as one of the most popular phylogenetic markers, our study indicated that ITS2 was a powerful barcode for Araliaceae identification. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：76 / 80

页数：5

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