Integration of organic electrochemical transistors and immuno-affinity membranes for label-free detection of interleukin-6 in the physiological concentration range through antibody-antigen recognition

被引：71

作者：

Gentili, Denis ^{[1
]}

D'Angelo, Pasquale ^{[2
]}

Militano, Francesca ^{[3
]}

Mazzei, Rosalinda ^{[3
]}

Poerio, Teresa ^{[3
]}

Brucale, Marco ^{[1
]}

Tarabella, Giuseppe ^{[2
,4
]}

Bonetti, Simone ^{[5
]}

Marasso, Simone L. ^{[2
,6
]}

Cocuzza, Matteo ^{[2
,6
]}

Giorno, Lidietta ^{[3
]}

Iannotta, Salvatore ^{[2
]}

Cavallini, Massimiliano ^{[1
]}

机构：

[1] CNR, ISMN, Via Gobetti 101, I-40129 Bologna, Italy

[2] CNR, IMEM, Parco Area Sci 37-A, I-43124 Parma, Italy

[3] CNR, ITM, Via Pietro Bucci 17-C, I-87036 Arcavacata Di Rende, Italy

[4] Camlin Italy Srl, Str Budellungo 2, I-43123 Parma, Italy

[5] CellDynam iSRL, Via P Gobetti 101, I-40129 Bologna, Italy

[6] Politecn Torino, Chilab Mat & Microsyst Lab, Dipartimento Sci Applicata & Tecnol DISAT, Via Lungo Piazza Armi 6, I-10034 Chivasso, Italy

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2018年 / 6卷 / 33期

关键词：

SELF-ASSEMBLED MONOLAYERS; IMPEDANCE SPECTROSCOPY; CAPACITIVE BIOSENSOR; GOLD; SURFACES; ENZYME; FUNCTIONALIZATION; FABRICATION; MODULATION; ADSORPTION;

D O I：

10.1039/c8tb01697f

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

We demonstrate the label-free and selective detection of interleukin-6 (IL-6), a key cell-signaling molecule in biology and medicine, by integrating an OECT with an immuno-affinity regenerated cellulose membrane. The objective of the membrane is to increase the local concentration of IL-6 at the sensing electrode and, thereby, enhance the device response for concentrations falling within the physiological concentration range of cytokines. The OECT gate electrode is functionalized with an oligo(ethylene glycol)-terminated self-assembled alkanethiolate monolayer (SAM) for both the immobilization of anti IL-6 antibodies and the inhibition of non-specific biomolecule binding. The OECT gate/electrolyte interface is exploited for the selective detection of IL-6 through the monitoring of antigen-antibody binding events occurring at the gate electrode.

引用

页码：5400 / 5406

页数：7

共 46 条

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