TGF-β regulates Sca-1 expression and plasticity of pre-neoplastic mammary epithelial stem cells

被引：5
|
作者：
Remsik, Jan [1 ,2 ,3 ,5 ]
Pickova, Marketa [1 ,2 ,3 ]
Vacek, Ondrej [1 ,2 ,3 ]
Fedr, Radek [1 ,2 ]
Bino, Lucia [1 ,2 ]
Hampl, Ales [2 ,4 ]
Soucek, Karel [1 ,2 ,3 ]
机构：
[1] Czech Acad Sci, Inst Biophys, Kralovopolska 135, Brno 61265, Czech Republic
[2] St Annes Univ Hosp Brno, Ctr Biomol & Cellular Engn, Int Clin Res Ctr, Pekarska 53, Brno 65691, Czech Republic
[3] Masaryk Univ, Fac Sci, Dept Expt Biol, Kamenice 5, Brno 62500, Czech Republic
[4] Masaryk Univ, Fac Med, Dept Histol & Embryol, Kamenice 5, Brno 62500, Czech Republic
[5] Mem Sloan Kettering Canc Ctr, Human Oncol & Pathogenesis Progra, New York, NY 10065 USA
来源：
SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期
关键词：
DIFFERENTIATION; IDENTIFICATION; PROMOTES; STATE;
D O I：
10.1038/s41598-020-67827-4
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The epithelial-mesenchymal plasticity, in tight association with stemness, contributes to the mammary gland homeostasis, evolution of early neoplastic lesions and cancer dissemination. Focused on cell surfaceome, we used mouse models of pre-neoplastic mammary epithelial and cancer stem cells to reveal the connection between cell surface markers and distinct cell phenotypes. We mechanistically dissected the TGF-beta family-driven regulation of Sca-1, one of the most commonly used adult stem cell markers. We further provided evidence that TGF-beta disrupts the lineage commitment and promotes the accumulation of tumor-initiating cells in pre-neoplastic cells.
引用
收藏
页数：12
相关论文
共 33 条
  • [21] TGF-β/Smad Signalling Activation by HTRA1 Regulates the Function of Human Lens Epithelial Cells and Its Mechanism in Posterior Subcapsular Congenital Cataract
    Lin, Xiaolei
    Yang, Tianke
    Liu, Xin
    Fan, Fan
    Zhou, Xiyue
    Li, Hongzhe
    Luo, Yi
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (22)
  • [22] Kruppel-like factor 4 regulates stemness and mesenchymal properties of colorectal cancer stem cells through the TGF-β1/Smad/snail pathway
    Leng, Zhengwei
    Li, Yong
    Zhou, Guojun
    Lv, Xiaojiang
    Ai, Walden
    Li, Jianshui
    Hou, Lingmi
    JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020, 24 (02) : 1866 - 1877
  • [23] The cooperative effects of micro-grooved topography and TGF-β1 on the vascular smooth muscle cell contractile protein expression of the mesenchymal stem cells
    Abolhasani, Sakhavat
    Rajabibazl, Masoumeh
    Khani, Mohammad-Mehdi
    Parandakh, Azim
    Hoseinpoor, Reyhaneh
    DIFFERENTIATION, 2020, 115 : 22 - 29
  • [24] Functional expression of smooth muscle-specific ion channels in TGF-β1-treated human adipose-derived mesenchymal stem cells
    Park, Won Sun
    Heo, Soon Chul
    Jeon, Eun Su
    Hong, Da Hye
    Son, Youn Kyoung
    Ko, Jae-Hong
    Kim, Hyoung Kyu
    Lee, Sun Young
    Kim, Jae Ho
    Han, Jin
    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2013, 305 (04): : C377 - C391
  • [25] Morphology and contractile gene expression of adipose-derived mesenchymal stem cells in response to short-term cyclic uniaxial strain and TGF-β1
    Rashidi, Neda
    Tafazzoli-Shadpour, Mohammad
    Haghighipour, Nooshin
    Khani, Mohammad-Mehdi
    BIOMEDICAL ENGINEERING-BIOMEDIZINISCHE TECHNIK, 2018, 63 (03): : 317 - 326
  • [26] Induced Pluripotent Stem Cells Inhibit Bleomycin-Induced Pulmonary Fibrosis in Mice through Suppressing TGF-β1/Smad-Mediated Epithelial to Mesenchymal Transition
    Zhou, Yan
    He, Zhong
    Gao, Yuan
    Zheng, Rui
    Zhang, Xiaoye
    Zhao, Li
    Tan, Mingqi
    FRONTIERS IN PHARMACOLOGY, 2016, 7
  • [27] TGF-β1 pathway affects the protein expression of many signaling pathways, markers of liver cancer stem cells, cytokeratins, and TERT in liver cancer HepG2 cells
    Wang, Xin-Hong
    Liu, Ming-Na
    Sun, Xun
    Xu, Chun-Huan
    Liu, Jing
    Chen, Jing
    Xu, Rui-Ling
    Li, Bao-Xin
    TUMOR BIOLOGY, 2016, 37 (03) : 3675 - 3681
  • [28] High Glucose Regulates Cyclin D1/E of Human Mesenchymal Stem Cells Through TGF-β1 Expression via Ca2+/PKC/MAPKs and PI3K/Akt/mTOR Signal Pathways
    Ryu, Jung Min
    Lee, Min Young
    Yun, Seung Pil
    Han, Ho Jae
    JOURNAL OF CELLULAR PHYSIOLOGY, 2010, 224 (01) : 59 - 70
  • [29] Short communication: Protein kinase C regulates glucose uptake and mRNA expression of glucose transporter (GLUT) 1 and GLUT8 in lactating bovine mammary epithelial cells
    Zhao, K.
    Liu, H. -Y.
    Zhao, F. -Q.
    Liu, J. -X.
    JOURNAL OF DAIRY SCIENCE, 2014, 97 (07) : 4329 - 4332
  • [30] TGF-β2-induced circ-PRDM5 regulates migration, invasion, and EMT through the miR-92b-3p/COL1A2 pathway in human lens epithelial cells
    Huang, Pengcheng
    Hu, Yao
    Duan, Yuping
    JOURNAL OF MOLECULAR HISTOLOGY, 2022, 53 (02) : 309 - 320
1234