The effect of purmorphamine on differentiation of endometrial stem cells into osteoblast-like cells on collagen/hydroxyapatite scaffolds

被引：14

作者：

Bahrami, Naghmeh ^{[1
,2
,3
,4
]}

Malekolkottab, Fatemeh ^{[5
]}

Ebrahimi-Barough, Somayeh ^{[6
]}

Tabari, Zahra Alizadeh ^{[5
]}

Hamisi, Jalaleddin ^{[5
]}

Kamyab, Ahmadreza ^{[7
]}

Mohamadnia, Abdolreza ^{[8
,9
]}

Ai, Armin ^{[1
]}

Bayat, Farshad ^{[1
]}

Bahrami, Naeim ^{[10
]}

Ai, Jafar ^{[6
]}

机构：

[1] Univ Tehran Med Sci, Sch Dent, Dept Oral & Maxillofacial Surg, Tehran, Iran

[2] Univ Tehran Med Sci, Craniomaxillo Facial Res Ctr, Tehran, Iran

[3] Univ Tehran Med Sci, Iranian Tissue Bank, Tehran, Iran

[4] Univ Tehran Med Sci, Res Ctr, Tehran, Iran

[5] Qazvin Univ Med Sci, Sch Dent, Dept Periodont, Qazvin, Iran

[6] Univ Tehran Med Sci, Fac Adv Technol Med, Dept Tissue Engn & Appl Cell Sci, Tehran, Iran

[7] Islamic Azad Univ, Sci & Res Branch, Dept Genet, Tehran, Iran

[8] Shahid Beheshti Univ Med Sci, NRITLD, Virol Res Ctr, Tehran, Iran

[9] Shahid Beheshti Univ Med Sci, Sch Adv Technol Med, Dept Biotechnol, Tehran, Iran

[10] Wake Forest Univ, Dept Biomed Engn, Virginia Tech, Winston Salem, NC 27109 USA

来源：

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2017年 / 45卷 / 07期

关键词：

hEnSCs; bone tissue engineering; collagen/hydroxyapatite scaffold; purmorphamin; REGENERATION; OSTEOGENESIS;

D O I：

10.1080/21691401.2016.1236804

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We assessed the effect of purmorphamine along with collagen/hydroxyapatite scaffold in inducing osteogenesis of human endometrial stem cells (hEnSCs). The adhesion, viability, proliferation, and differentiation of cells on scaffold were assayed with SEM, MTT, real time-PCR, and ALP assay, respectively. The results were shown good integration of cells with scaffold. Also, qRT-PCR of differentiated cells shows that osteoblast cell markers are expressed after 21d in 2D and scaffold groups while in the scaffold group the expression of these markers were higher than the 2D group. Based on our findings, collagen/hydroxyapatite scaffold with PMA has the potential role in osteogenic differentiation of hEnSCs.

引用

页码：1343 / 1349

页数：7

共 33 条

[11] The effect of purmorphamine and sirolimus on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells [J].

Faghihi, F. ;

Eslaminejad, M. Baghaban ;

Nekookar, A. ;

Najar, M. ;

Salekdeh, G. H. .

BIOMEDICINE & PHARMACOTHERAPY, 2013, 67 (01) :31-38

[12] Effect of Purmorphamine on Osteogenic Differentiation of Human Mesenchymal Stem Cells in a Three-Dimensional Dynamic Culture System [J].

Faghihi, Faezeh ;

Papadimitropoulos, Adam ;

Martin, Ivan ;

Eslaminejad, Mohamadreza Baghaban .

CELLULAR AND MOLECULAR BIOENGINEERING, 2014, 7 (04) :575-584

[13] Endometrial regeneration and endometrial stem/progenitor cells [J].

Gargett, Caroline E. ;

Nguyen, Hong P. T. ;

Ye, Louie .

REVIEWS IN ENDOCRINE & METABOLIC DISORDERS, 2012, 13 (04) :235-251

[14] Adult stem cells in the endometrium [J].

Gargett, Caroline E. ;

Masuda, Hirotaka .

MOLECULAR HUMAN REPRODUCTION, 2010, 16 (11) :818-834

[15] Isolation and Culture of Epithelial Progenitors and Mesenchymal Stem Cells from Human Endometrium [J].

Gargett, Caroline E. ;

Schwab, Kjiana E. ;

Zillwood, Rachel M. ;

Nguyen, Hong P. T. ;

Wu, Di .

BIOLOGY OF REPRODUCTION, 2009, 80 (06) :1136-1145

[16]

Gleeson JP, 2010, EUR CELLS MATER, V20, P218

[17] Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow [J].

Gronthos, S ;

Zannettino, ACW ;

Hay, SJ ;

Shi, ST ;

Graves, SE ;

Kortesidis, A ;

Simmons, PJ .

JOURNAL OF CELL SCIENCE, 2003, 116 (09) :1827-1835

[18] Neuroprotective Effect of Transplanted Neural Precursors Embedded on PLA/CS Scaffold in an Animal Model of Multiple Sclerosis [J].

Hoveizi, Elham ;

Tavakol, Shima ;

Ebrahimi-Barough, Somayeh .

MOLECULAR NEUROBIOLOGY, 2015, 51 (03) :1334-1342

[19] Clinical utility of stem cells for periodontal regeneration [J].

Hynes, Kim ;

Menicanin, Danijela ;

Gronthos, Stan ;

Bartold, P. Mark .

PERIODONTOLOGY 2000, 2012, 59 :203-227

[20] The application of bone morphogenetic proteins to dental tissue engineering [J].

Nakashima, M ;

Reddi, AH .

NATURE BIOTECHNOLOGY, 2003, 21 (09) :1025-1032

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