The effect of purmorphamine on differentiation of endometrial stem cells into osteoblast-like cells on collagen/hydroxyapatite scaffolds

被引：14

作者：

Bahrami, Naghmeh ^{[1
,2
,3
,4
]}

Malekolkottab, Fatemeh ^{[5
]}

Ebrahimi-Barough, Somayeh ^{[6
]}

Tabari, Zahra Alizadeh ^{[5
]}

Hamisi, Jalaleddin ^{[5
]}

Kamyab, Ahmadreza ^{[7
]}

Mohamadnia, Abdolreza ^{[8
,9
]}

Ai, Armin ^{[1
]}

Bayat, Farshad ^{[1
]}

Bahrami, Naeim ^{[10
]}

Ai, Jafar ^{[6
]}

机构：

[1] Univ Tehran Med Sci, Sch Dent, Dept Oral & Maxillofacial Surg, Tehran, Iran

[2] Univ Tehran Med Sci, Craniomaxillo Facial Res Ctr, Tehran, Iran

[3] Univ Tehran Med Sci, Iranian Tissue Bank, Tehran, Iran

[4] Univ Tehran Med Sci, Res Ctr, Tehran, Iran

[5] Qazvin Univ Med Sci, Sch Dent, Dept Periodont, Qazvin, Iran

[6] Univ Tehran Med Sci, Fac Adv Technol Med, Dept Tissue Engn & Appl Cell Sci, Tehran, Iran

[7] Islamic Azad Univ, Sci & Res Branch, Dept Genet, Tehran, Iran

[8] Shahid Beheshti Univ Med Sci, NRITLD, Virol Res Ctr, Tehran, Iran

[9] Shahid Beheshti Univ Med Sci, Sch Adv Technol Med, Dept Biotechnol, Tehran, Iran

[10] Wake Forest Univ, Dept Biomed Engn, Virginia Tech, Winston Salem, NC 27109 USA

来源：

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2017年 / 45卷 / 07期

关键词：

hEnSCs; bone tissue engineering; collagen/hydroxyapatite scaffold; purmorphamin; REGENERATION; OSTEOGENESIS;

D O I：

10.1080/21691401.2016.1236804

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We assessed the effect of purmorphamine along with collagen/hydroxyapatite scaffold in inducing osteogenesis of human endometrial stem cells (hEnSCs). The adhesion, viability, proliferation, and differentiation of cells on scaffold were assayed with SEM, MTT, real time-PCR, and ALP assay, respectively. The results were shown good integration of cells with scaffold. Also, qRT-PCR of differentiated cells shows that osteoblast cell markers are expressed after 21d in 2D and scaffold groups while in the scaffold group the expression of these markers were higher than the 2D group. Based on our findings, collagen/hydroxyapatite scaffold with PMA has the potential role in osteogenic differentiation of hEnSCs.

引用

页码：1343 / 1349

页数：7

共 33 条

[1] Osteoinductive Small Molecules: Growth Factor Alternatives for Bone Tissue Engineering
Aravamudhan, Aja
Ramos, Daisy M.
Nip, Jonathan
Subramanian, Aditi
James, Roshan
Harmon, Matthew D.
Yu, Xiaojun
Kumbar, Sangamesh G.
[J]. CURRENT PHARMACEUTICAL DESIGN, 2013, 19 (19) : 3420 - 3428
[2] Expansion and Characterization of Human Embryonic Stem Cell-Derived Osteoblast-Like Cells
Arpornmaeklong, Premjit
Wang, Zhuo
Pressler, Michael J.
Brown, Shelley E.
Krebsbach, Paul H.
[J]. CELLULAR REPROGRAMMING, 2010, 12 (04) : 377 - 389
[3] In vitro evaluation of biomimetic nanocomposite scaffold using endometrial stem cell derived osteoblast-like cells
Azami, Mahmoud
Ai, Jafar
Ebrahimi-Barough, Somayeh
Farokhi, Mehdi
Fard, Sahar E.
[J]. TISSUE & CELL, 2013, 45 (05) : 328 - 337
[4] Bone sialoprotein-collagen interaction promotes hydroxyapatite nucleation
Baht, Gurpreet S.
Hunter, Graeme K.
Goldberg, Harvey A.
[J]. MATRIX BIOLOGY, 2008, 27 (07) : 600 - 608
[5] Principles and applications of cell delivery systems for periodontal regeneration
Bartold, PM
Xiao, Y
Lyngstaadas, SP
Paine, ML
Snead, ML
[J]. PERIODONTOLOGY 2000, 2006, 41 : 123 - 135
[6] Purmorphamine enhances osteogenic activity of human osteoblasts derived from bone marrow mesenchymal cells
Beloti, MM
Bellesini, LS
Rosa, AL
[J]. CELL BIOLOGY INTERNATIONAL, 2005, 29 (07) : 537 - 541
[7] Campos Doris M, 2013, Biomatter, V3, DOI 10.4161/biom.24922
[8] Clonogenicity of human endometrial epithelial and stromal cellss
Chan, RWS
Schwab, KE
Gargett, CE
[J]. BIOLOGY OF REPRODUCTION, 2004, 70 (06) : 1738 - 1750
[9] Investigating the neuroglial differentiation effect of neuroblastoma conditioned medium in human endometrial stem cells cultured on 3D nanofibrous scaffold
Ebrahimi-Barough, Somayeh
Hoveizi, Elham
Javidan, Abbas Norouzi
Ai, Jafar
[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2015, 103 (08) : 2621 - 2627
[10] Differentiation of Human Endometrial Stromal Cells into Oligodendrocyte Progenitor Cells (OPCs)
Ebrahimi-Barough, Somayeh
Kouchesfahani, Homa Mohseni
Ai, Jafar
Massumi, Mohammad
[J]. JOURNAL OF MOLECULAR NEUROSCIENCE, 2013, 51 (02) : 265 - 273

← 1 2 3 4 →