Induction of endophytic colonization in rice (Oryza sativa L.) tissue culture plants by Azorhizobium caulinodans

被引：11
|
作者：
Senthilkumar, M. [1 ,2 ]
Madhaiyan, M. [3 ,4 ]
Sundaram, S. P. [4 ]
Sangeetha, H. [4 ]
Kannaiyan, S. [5 ]
机构：
[1] Tamil Nadu Agr Univ, Hort Coll, Dept Agr Microbiol, Periyakulam 625604, Tamil Nadu, India
[2] Tamil Nadu Agr Univ, Res Inst, Periyakulam 625604, Tamil Nadu, India
[3] Chungbuk Natl Univ, Dept Agr Chem, Cheongju 361763, Chungbuk, South Korea
[4] Tamil Nadu Agr Univ, Dept Agr Microbiol, Coimbatore 641003, Tamil Nadu, India
[5] Natl Biodivers Author India, Madras, Tamil Nadu, India
来源：
BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 08期
关键词：
rice; callus induction; Azorhizobium; endophytic colonization; Aeschynomene;
D O I：
10.1007/s10529-008-9693-6
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Endophytic colonization in rice was induced using rhizobia. Dehusked seeds of rice hybrid, CORH2, were used as explants for induction of calli. MS medium was modified with 2,4-D (2.5 mg l(-1)) and kinetin (0.2 mg l(-1)) for callus induction. Well-developed calli were inoculated with Azorhizobium caulinodans strains ORS 571 and AA-SK-5 by means of imbibition. All treated calli had significant increases in protein content, total nitrogen and nitrogenase activity. Imbibition of ORS 571 had significant biochemical effect on the developing calli than AA-SK-5. The crop response study from the regenerated plantlets showed a positive correlation in yield than uninoculated control. The endophytic colonization was observed in all parts of the plants analyzed. Further, colonization was also confirmed by microtome sectioning.
引用
收藏
页码：1477 / 1487
页数：11
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