Investigation of the antigenic evolution of field isolates using the reverse genetics system of infectious bursal disease virus (IBDV)

被引：30
|
作者：
Durairaj, Vijay [1 ]
Sellers, Holly S. [1 ]
Linnemann, Erich G. [1 ]
Icard, Alan H. [1 ]
Mundt, Egbert [1 ]
机构：
[1] Univ Georgia, Coll Vet Med, Poultry Diagnost & Res Ctr, Athens, GA 30602 USA
来源：
ARCHIVES OF VIROLOGY | 2011年 / 156卷 / 10期
关键词：
AMINO-ACIDS; NUCLEOTIDE-SEQUENCES; DIFFERENT STRAINS; ESCHERICHIA-COLI; GENOMIC SEGMENT; MOLECULAR-BASIS; SEROTYPES; VP2; GENE; RNA; DIFFERENTIATION;
D O I：
10.1007/s00705-011-1040-x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The antigenic profiles of over 300 infectious bursal disease virus (IBDV) isolates were analyzed using a panel of monoclonal antibodies in a reverse genetics system. In addition, the sequences of a large portion of the neutralizing-antibody-inducing VP2 of IBDV were determined. Phylogenetic analysis of nucleotide and amino acid sequences in combination with the antigenic profiles obtained using the monoclonal antibody panel, revealed a lack of correlation between antigenicity and isolate's placement within the phylogenetic tree. In-depth analysis of amino acid exchanges revealed that changes within a certain region of the VP2 molecule resulted in differences in the antigenicity of the virus. This comprehensive analysis of VP2 sequences indicated a high selective pressure in the field that was likely due to vaccination programs, which increase the rate of evolution of the virus.
引用
收藏
页码：1717 / 1728
页数：12
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