Functional characterization, secondary structure prediction and analysis of ectoine biosynthesis genes from Bacillus halodurans: an osmolyte involved in stress tolerance

被引：0
|
作者：
Rajan, Lawrance Anbu [1 ]
Joseph, Toms C. [1 ]
Thampuran, Nirmala [1 ]
James, Roswin [1 ]
机构：
[1] Cent Inst Fisheries Technol, Microbiol Fermentat & Biotechnol Div, Cochin 682029, Kerala, India
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2010年 / 26卷 / 11期
关键词：
Bacillus halodurans; Compatible solutes; Ectoine genes; Osmoregulation; Salt stress; COMPATIBLE SOLUTE ECTOINE;
D O I：
10.1007/s11274-010-0379-6
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An open reading frame encoding the ectoine biosynthesis genes was cloned from the Bacillus halodurans genome. An expression plasmid containing the operon was introduced into Escherichia coli cells, and the recombinant ectoine was quantified. The secondary structure of ectoine biosynthesis proteins were predicted and was quite similar to that of reported proteins from eubacteria.
引用
收藏
页码：2093 / 2098
页数：6
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