pDNA loaded calcium phosphate nanoparticles: highly efficient non-viral vector for gene delivery

被引：195

作者：

Bisht, S ^{[1
]}

Bhakta, G ^{[1
]}

Mitra, S ^{[1
]}

Maitra, A ^{[1
]}

机构：

[1] Univ Delhi, Dept Chem, Delhi 110007, India

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2005年 / 288卷 / 01期

关键词：

calcium phosphate nanoparticles; non-viral vectors; gene delivery; transfection efficiency;

D O I：

10.1016/j.ijpharm.2004.07.035

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Nanoparticles of calcium phosphate encapsulating plasmid DNA (pDNA) of size 100-120nm in diameter were prepared. XRD studies of these nanoparticles showed them to be crystalline in nature having hydroxyapatite structure. The maximum loading of pDNA and its release from nanoparticles were studied using gel electrophoresis. The time dependent size measurement of these particles demonstrated that these particles show strong aggregational behaviour in aqueous dispersion. Calcium phosphate nanoparticles were found to be dissolved even in low acidic buffer (pH 5.0) releasing the pDNA, which suggested that DNA release from these particles in the endosomal compartment was possible. In vitro transfection efficiency of these calcium phosphate nanoparticles was found to be higher than that of the commercial transfecting reagent Polyfect. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：157 / 168

页数：12

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