Inhibition of viral adsorption and penetration by an aqueous extract from Rhododendron ferrugineum L. as antiviral principle against herpes simplex virus type-1

被引：48

作者：

Gescher, Kirsten ^{[1
]}

Kuehn, Joachim ^{[2
]}

Hafezi, Wali ^{[2
]}

Louis, Andreas ^{[1
]}

Derksen, Andrea ^{[1
]}

Deters, Alexandra ^{[1
]}

Lorentzen, Eva ^{[2
]}

Hensel, Andreas ^{[1
]}

机构：

[1] Univ Munster, Inst Pharmaceut Biol & Phytochem, D-48149 Munster, Germany

[2] Univ Hosp Munster, Inst Med Microbiol, D-48151 Munster, Germany

来源：

FITOTERAPIA | 2011年 / 82卷 / 03期

关键词：

Rhododendron ferrugineum L; Ericaceae; Herpes simplex virus type-1; Attachment; Penetration; gD protein; COLORIMETRIC ASSAY; REPLICATION; INVITRO; GALLATE; ENTRY;

D O I：

10.1016/j.fitote.2010.11.022

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The polyphenol-enriched aqueous extract RF from the aerial parts of Rhododendron ferrugineum exhibited strong antiviral activity against herpes simplex virus type-1 while adenovirus 3 was not affected. RF exhibited an IC50 of 7.4 mu g/mL and a selectivity index of 64 when added to the virus inoculum prior to infection. RF abolished virus entry into the host cell by blocking attachment to the cell surface. When added after attachment at a concentration of > 25 mu g/mL, RF inhibited also penetration of HSV-1 into the host cell. RF directly interacts with viral envelope proteins as demonstrated for the viral glycoprotein gD. (c) 2010 Elsevier B.V. All rights reserved.

引用

页码：408 / 413

页数：6

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