Effects of IL-12 and IL-18 on HBcAg-specific cytokine production by CD4 T lymphocytes of children with chronic hepatitis B infection

被引：36

作者：

Szkaradkiewicz, A ^{[1
]}

Jopek, A ^{[1
]}

Wysocki, J ^{[1
]}

机构：

[1] Poznan Univ Med Sci, Poznan, Poland

来源：

ANTIVIRAL RESEARCH | 2005年 / 66卷 / 01期

关键词：

hepatitis B virus; Th lymphocytes; interferon-gamma; interleukin-12; interlenkin-18;

D O I：

10.1016/j.antiviral.2004.12.005

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The influence of IL-12 and IL-18 was evaluated on hepatitis B core antigen (HBcAg)-specific cytokine production (IFN-gamma, IL-4, IL-5 and IL-10) by CD4 T lymphocytes isolated from peripheral blood of children with chronic hepatitis B. CD4 T cells were isolated from peripheral blood of 20 children with chronic active hepatitis B, cultured for 48 h in presence of rHBcAg and of co-stimulators, IL-12 or IL-18 or IL-12 + IL-18 or in their absence (control). Production of studied cytokines was examined using the ELISPOT assay. Co-stimulation with IL-12 or IL-18 was found to significantly augment the HBcAg-specific secretion of IFN-gamma. However, the most pronounced stimulatory effect was observed in the presence of IL-12 + IL-18 and resulted in peak levels of IFN-gamma production. The obtained results allowed concluding that the anti-HBV activity of Th1 lymphocytes is strongly induced by IL-12 + IL-18 and may contribute to viral clearance in children with chronic hepatitis B infection. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：23 / 27

页数：5

共 50 条

[1] HBcAg-specific cytokine production by CD4 T lymphocytes of children with acute and chronic hepatitis B
Szkaradkiewicz, A
Jopek, A
Wysocki, J
Grzymislawski, M
Malecka, I
Wozniak, A
VIRUS RESEARCH, 2003, 97 (02) : 127 - 133
[2] Expression of the cytokine receptors for IL-12 and IL-18 on naive and memory CD4+T cells
Bofill, M
Pena, R
Ruiz-Hernandez, R
Naranjo, M
Clotet, B
Borras, F
IMMUNOLOGY 2004: CYTOKINE NETWORK, REGULATORY CELLS, SIGNALING, AND APOPTOSIS, 2004, : 25 - 28
[3] IL-18, but not IL-12, is required for optimal cytokine production by influenza virus-specific CD8+ T cells
Denton, Alice E.
Doherty, Peter C.
Turner, Stephen J.
La Gruta, Nicole L.
EUROPEAN JOURNAL OF IMMUNOLOGY, 2007, 37 (02) : 368 - 375
[4] IL-12 INHIBITS THE PRODUCTION OF IL-4 AND IL-10 IN ALLERGEN-SPECIFIC HUMAN CD4(+) T-LYMPHOCYTES
MARSHALL, JD
SECRIST, H
DEKRUYFF, RH
WOLF, SF
UMETSU, DT
JOURNAL OF IMMUNOLOGY, 1995, 155 (01): : 111 - 117
[5] Effects of IL-12 and IL-18 on the cytotoxic activities of intraepithelial lymphocytes (IELs)
Ebert, EC
Roberts, AI
GASTROENTEROLOGY, 2001, 120 (05) : A320 - A320
[6] Combined effects of IL-12 and IL-18 on the clinical course and local cytokine production in murine pulmonary infection with Cryptococcos neoformans
Qureshi, MH
Zhang, TT
Koguchi, Y
Nakashima, K
Okamura, H
Kurimoto, M
Kawakami, K
EUROPEAN JOURNAL OF IMMUNOLOGY, 1999, 29 (02) : 643 - 649
[7] HBcAg-Specific IL-21-Producing CD4+T Cells are Associated with Relative Viral Control in Patients with Chronic Hepatitis B
Li, L.
Liu, M.
Cheng, L. -W.
Gao, X. -Y.
Fu, J. -J.
Kong, G.
Feng, X.
Pan, X. -C.
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2013, 78 (05) : 439 - 446
[8] Production of IL-12 and IL-18 in human dendritic cells upon infection by Listeria monocytogenes
Kolb-Mäurer, A
Kämmerer, U
Mäurer, M
Gentschev, I
Bröcker, EB
Rieckmann, P
Kämpgen, E
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2003, 35 (03): : 255 - 262
[9] INTERLEUKIN (IL)-12 INHIBITS THE PRODUCTION OF IL-4 AND IL-10 IN ALLERGEN-SPECIFIC HUMAN CD4(+) T-LYMPHOCYTES
MARSHALL, JD
SECRIST, H
DEKRUYFF, RH
WOLF, SF
UMETSU, DT
FASEB JOURNAL, 1995, 9 (03): : A204 - A204
[10] Activation of virus-specific CD8+ T cells by lipopolysaccharide-induced IL-12 and IL-18
Raué, HP
Brien, JD
Hammarlund, E
Slifka, MK
JOURNAL OF IMMUNOLOGY, 2004, 173 (11): : 6873 - 6881

← 1 2 3 4 5 →