Effects of IL-12 and IL-18 on HBcAg-specific cytokine production by CD4 T lymphocytes of children with chronic hepatitis B infection

被引：36

作者：

Szkaradkiewicz, A ^{[1
]}

Jopek, A ^{[1
]}

Wysocki, J ^{[1
]}

机构：

[1] Poznan Univ Med Sci, Poznan, Poland

来源：

ANTIVIRAL RESEARCH | 2005年 / 66卷 / 01期

关键词：

hepatitis B virus; Th lymphocytes; interferon-gamma; interleukin-12; interlenkin-18;

D O I：

10.1016/j.antiviral.2004.12.005

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The influence of IL-12 and IL-18 was evaluated on hepatitis B core antigen (HBcAg)-specific cytokine production (IFN-gamma, IL-4, IL-5 and IL-10) by CD4 T lymphocytes isolated from peripheral blood of children with chronic hepatitis B. CD4 T cells were isolated from peripheral blood of 20 children with chronic active hepatitis B, cultured for 48 h in presence of rHBcAg and of co-stimulators, IL-12 or IL-18 or IL-12 + IL-18 or in their absence (control). Production of studied cytokines was examined using the ELISPOT assay. Co-stimulation with IL-12 or IL-18 was found to significantly augment the HBcAg-specific secretion of IFN-gamma. However, the most pronounced stimulatory effect was observed in the presence of IL-12 + IL-18 and resulted in peak levels of IFN-gamma production. The obtained results allowed concluding that the anti-HBV activity of Th1 lymphocytes is strongly induced by IL-12 + IL-18 and may contribute to viral clearance in children with chronic hepatitis B infection. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：23 / 27

页数：5

共 23 条

[1] Interleukin-12 inhibits hepatitis B virus replication in transgenic mice [J].