A validated GC-MS method for the determination of Δ9-tetrahydrocannabinol and 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid in bile samples

被引：8

作者：

Papoutsis, Ioannis ^{[1
]}

Nikolaou, Panagiota ^{[1
]}

Dona, Artemisia ^{[1
]}

Pistos, Constantinos ^{[1
]}

Stefanidou, Maria ^{[1
]}

Spiliopoulou, Chara ^{[1
]}

Athanaselis, Sotirios ^{[1
]}

机构：

[1] Univ Athens, Dept Forens Med & Toxicol, Sch Med, Athens 11527, Greece

来源：

FORENSIC TOXICOLOGY | 2012年 / 30卷 / 01期

关键词：

Delta(9)-Tetrahydrocannabinol; 11-Nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid; Bile; GC-MS; SOLID-PHASE EXTRACTION; 2-DIMENSIONAL GAS-CHROMATOGRAPHY; WHOLE-BLOOD; LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; HUMAN PLASMA; CANNABINOIDS; THC; URINE; TETRAHYDROCANNABINOL;

D O I：

10.1007/s11419-011-0126-1

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

We present a validated gas chromatography-mass spectrometry method for the determination of Delta(9)-tetrahydrocannabinol and 11-nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid in bile samples. The method includes protein precipitation with acetonitrile after enzymatic hydrolysis, and solid-phase extraction followed by silylation using N,O-bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane. The limit of detection was 0.30 ng/ml and the limit of quantitation was 1.00 ng/ml. The calibration curves were linear within the dynamic range of 1.00-500 ng/ml (R (2) a parts per thousand yen 0.993) and the absolute recovery for both analytes was higher than 87.5%. Accuracy and precision were less than 8.8% and 8.2%, respectively. The developed method was applied for the analysis of bile samples obtained from 21 forensic cases.

引用

页码：51 / 58

页数：8

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