Surface modification of polydimethylsiloxane with photo-grafted poly(ethylene glycol) for micropatterned protein adsorption and cell adhesion

被引：82

作者：

Sugiura, Shinji ^{[1
]}

Edahiro, Jun-ichi ^{[1
]}

Sumaru, Kimio ^{[1
]}

Kanamori, Toshiyuki ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Res Ctr Adv Bion, Tsukuba, Ibaraki 3058565, Japan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2008年 / 63卷 / 02期

关键词：

poly(ethylene glycol); photo-induced graft polymerization; protein micropatterning; cell micropatterning; polydimethylsiloxane;

D O I：

10.1016/j.colsurfb.2007.12.013

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this study, we applied photo-induced graft polymerization to micropatterned surface modification of polydimethylsiloxane (PDMS) with poly(ethylene glycol). Two types of monomers, polyethylene glycol monoacrylate (PEGMA) and polyethylene glycol diacrylate (PEGDA), were tested for surface modification of PDMS. Changes in the surface hydrophilicity and surface element composition were characterized by contact angle measurement and electron spectroscopy for chemical analysis. The PEGMA-grafted PDMS surfaces gradually lost their hydrophilicity within two weeks. In contrast, the PEGDA-grafted PDMS surface maintained stable hydrophilic characteristics for more than two months. Micropatterned protein adsorption and micropatterned cell adhesion were successfully demonstrated using PEGDA-micropatterned PDMS surfaces, which were prepared by photo-induced graft polymerization using photomasks. The PEGDA-grafted PDMS exhibited useful characteristics for microfluidic devices (e.g. hydrophilicity, low protein adsorption, and low cell attachment). The technique presented in this study will be useful for surface modification of various research tools and devices. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：301 / 305

页数：5

共 32 条

[1] Stable modification of PDMS surface properties by plasma polymerization: Application to the formation of double emulsions in microfluidic systems [J].

Barbier, Valessa ;

Tatoulian, Michael ;

Li, Hong ;

Arefi-Khonsari, Farzaneh ;

Ajdari, Armand ;

Tabeling, Patrick .

LANGMUIR, 2006, 22 (12) :5230-5232

[2] Protein repellant silicone surfaces by covalent immobilization of poly(ethylene oxide) [J].

Chen, H ;

Zhang, Z ;

Chen, Y ;

Brook, MA ;

Sheardown, H .

BIOMATERIALS, 2005, 26 (15) :2391-2399

[3]

CHEN H, 2008, COLLOIDS SURF B BIOI, V61, P208

[4]

Delamarche E, 2001, ADV MATER, V13, P1164

[5] Rapid prototyping of microfluidic systems in poly(dimethylsiloxane) [J].

Duffy, DC ;

McDonald, JC ;

Schueller, OJA ;

Whitesides, GM .

ANALYTICAL CHEMISTRY, 1998, 70 (23) :4974-4984

[6] Surface engineering approaches to micropattern surfaces for cell-based assays [J].

Falconnet, D ;

Csucs, G ;

Grandin, HM ;

Textor, M .

BIOMATERIALS, 2006, 27 (16) :3044-3063

[7] A combined photolithographic and molecular-assembly approach to produce functional micropatterns for applications in the biosciences [J].

Falconnet, D ;

Koenig, A ;

Assi, T ;

Textor, M .

ADVANCED FUNCTIONAL MATERIALS, 2004, 14 (08) :749-756

[8] Novel hepatocyte culture system developed using microfabrication and collagen/polyethylene glycol microcontact printing [J].

Fukuda, J ;

Sakai, Y ;

Nakazawa, K .

BIOMATERIALS, 2006, 27 (07) :1061-1070

[9] Preparation of hydrophilic poly(dimethylsiloxane) stamps by plasma-induced grafting [J].

He, QG ;

Liu, ZC ;

Xiao, PF ;

Liang, RQ ;

He, NY ;

Lu, ZH .

LANGMUIR, 2003, 19 (17) :6982-6986

[10] Poly(oxyethylene) based surface coatings for poly(dimethylsiloxane) microchannels [J].

Hellmich, W ;

Regtmeier, J ;

Duong, TT ;

Ros, R ;

Anselmetti, D ;

Ros, A .

LANGMUIR, 2005, 21 (16) :7551-7557

← 1 2 3 4 →