Electrochemical method for isolation of chitinous 3D scaffolds from cultivated Aplysina aerophoba marine demosponge and its biomimetic application

被引：21

作者：

Nowacki, Krzysztof ^{[1
]}

Stepniak, Izabela ^{[1
]}

Machalowski, Tomasz ^{[2
,3
]}

Wysokowski, Marcin ^{[2
,3
]}

Petrenko, Iaroslav ^{[3
]}

Schimpf, Christoph ^{[4
]}

Rafaja, David ^{[4
]}

Langer, Enrico ^{[5
]}

Richter, Andreas ^{[5
]}

Zietek, Jerzy ^{[6
,7
]}

Pantovic, Snezana ^{[8
]}

Voronkina, Alona ^{[9
]}

Kovalchuk, Valentine ^{[10
]}

Ivanenko, Viatcheslav ^{[11
,12
]}

Khrunyk, Yuliya ^{[13
,14
]}

Galli, Roberta ^{[15
]}

Joseph, Yvonne ^{[3
]}

Gelinsky, Michael ^{[16
,17
]}

Jesionowski, Teofil ^{[2
]}

Ehrlich, Hermann ^{[3
,18
]}

机构：

[1] Poznan Univ Tech, Inst Chem & Tech Electrochem, Ul Berdychowo 4, PL-60965 Poznan, Poland

[2] Poznan Univ Tech, Fac Chem Technol, Inst Chem Technol & Engn, Berdychowo 4, PL-60965 Poznan, Poland

[3] TU Bergakad Freiberg, Inst Elect & Sensor Mat, Gustav Zeuner Str 3, D-09599 Freiberg, Germany

[4] TU Bergakad Freiberg, Inst Mat Sci, D-09599 Freiberg, Germany

[5] Tech Univ Dresden, Inst Semicond & Microsyst, D-01062 Dresden, Germany

[6] Univ Life Sci, Dept Epizootiol, Fac Vet Med, Gleboka 30, PL-20612 Lublin, Poland

[7] Univ Life Sci, Clin Infect Dis, Fac Vet Med, Gleboka 30, PL-20612 Lublin, Poland

[8] Univ Montenegro, Fac Med, Podgorica 81000, Montenegro

[9] Natl Pirogov Mem Med Univ, Dept Pharm, UA-21018 Vinnytsia, Ukraine

[10] Natl Pirogov Mem Med Univ, Dept Microbiol, UA-21018 Vinnytsya, Vinnytsia, Ukraine

[11] Lomonosov Moscow State Univ, Biol Fac, Dept Invertebrate Zool, Moscow 119992, Russia

[12] Naturalis Biodivers Ctr, Taxon & Systemat Grp, NL-2300 RA Leiden, Netherlands

[13] Ural Fed Univ, Mira Str 19, Ekaterinburg 620002, Russia

[14] Russian Acad Sci, Ural Branch, Inst High Temp Electrochem, Akad Skaya Str 20, Ekaterinburg 620990, Russia

[15] Tech Univ Dresden, Fac Med, Dept Anesthesiol & Intens Care Med, Clin Sensoring & Monitoring, D-01307 Dresden, Germany

[16] Tech Univ Dresden, Univ Hosp Carl Gustav Carus, Ctr Translat Bone Joint Soft Tissue Res, Med Fac, D-01307 Dresden, Germany

[17] Tech Univ Dresden, Univ Hosp Carl Gustav Carus, Univ Ctr Orthopaed & Trauma Surg, D-01307 Dresden, Germany

[18] Adam Mickiewicz Univ, Ctr Adv Technol, PL-61614 Poznan, Poland

来源：

APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING | 2020年 / 126卷 / 05期

基金：

俄罗斯科学基金会;

关键词：

Chitin; Scaffolds; Electrolysis; Biomineralization; Biomimetics; Hemolymph; Marine sponges; Aplysina aerophoba; SHELL-REPAIR MEMBRANE; CALCIUM-CARBONATE; CATION-EXCHANGE; PHYSICOCHEMICAL CHARACTERIZATION; AQUEOUS-SOLUTION; HEAVY-METALS; ELECTROLYSIS; CHITOSAN; REMOVAL; WATER;

D O I：

10.1007/s00339-020-03533-2

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Three-dimensional (3D) biopolymer-based scaffolds including chitinous matrices have been widely used for tissue engineering, regenerative medicine and other modern interdisciplinary fields including extreme biomimetics. In this study, we introduce a novel, electrochemically assisted method for 3D chitin scaffolds isolation from the cultivated marine demosponge Aplysina aerophoba which consists of three main steps: (1) decellularization, (2) decalcification and (3) main deproteinization along with desilicification and depigmentation. For the first time, the obtained electrochemically isolated 3D chitinous scaffolds have been further biomineralized ex vivo using hemolymph of Cornu aspersum edible snail aimed to generate calcium carbonates-based layered biomimetic scaffolds. The analysis of prior to, during and post-electrochemical isolation samples as well as samples treated with molluscan hemolymph was conducted employing analytical techniques such as SEM, XRD, ATR-FTIR and Raman spectroscopy. Finally, the use of described method for chitin isolation combined with biomineralization ex vivo resulted in the formation of crystalline (calcite) calcium carbonate-based deposits on the surface of chitinous scaffolds, which could serve as promising biomaterials for the wide range of biomedical, environmental and biomimetic applications.

引用

页数：16

共 115 条

[1]

Abolins-Krogis A., 1968, Symposia of the Zoological Society of London, VNo. 22, P75

[2] ULTRASTRUCTURAL-STUDY OF SHELL-REPAIR MEMBRANE IN SNAIL, HELIX-POMATIA L [J].

ABOLINSKROGIS, A .

CELL AND TISSUE RESEARCH, 1976, 172 (04) :455-476

[3] FLUORESCENCE AND HISTOCHEMICAL STUDIES OF CALCIFICATION-INITIATING LIPOFUSCIN TYPE PIGMENT GRANULES IN SHELL-REPAIR MEMBRANE OF SNAIL, HELIX-POMATIA L [J].

ABOLINSKROGIS, A .

ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE, 1973, 142 (02) :205-221

[4] Mollusk shell formation: A source of new concepts for understanding biomineralization processes [J].

Addadi, L ;

Joester, D ;

Nudelman, F ;

Weiner, S .

CHEMISTRY-A EUROPEAN JOURNAL, 2006, 12 (04) :981-987

[5]

[Anonymous], MED WETER

[6] The electrolytic production of acid and alkali from sodium sulfate solutions [J].

Atwell, HV ;

Fuwa, T .

INDUSTRIAL AND ENGINEERING CHEMISTRY, 1923, 15 :617-620

[7]

Auzoux-Bordenave S, 2007, J SHELLFISH RES, V26, P887, DOI 10.2983/0730-8000(2007)26[887:CRTAOM]2.0.CO

[8]

[9] Controllable Synthesis of Calcium Carbonate with Different Geometry: Comprehensive Analysis of Particle Formation, Cellular Uptake, and Biocompatibility [J].

Bahrom, Hani ;

Goncharenko, Alexander A. ;

Fatkhutdinova, Landysh I. ;

Peltek, Oleksii O. ;

Muslimov, Albert R. ;

Koval, Olga Yu. ;

Eliseev, Igor E. ;

Manchey, Andrey ;

Gorin, Dmitry ;

Shishkin, Ivan I. ;

Noskov, Roman E. ;

Timin, Alexander S. ;

Ginzburg, Pavel ;

Zyuzin, Mikhail V. .

ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 2019, 7 (23) :19142-19156

[10] Chitin Facilitated Mineralization in the Eastern Oyster [J].

Bin San Chan, Vera ;

Johnstone, Mary Beth ;

Wheeler, A. P. ;

Mount, Andrew S. .

FRONTIERS IN MARINE SCIENCE, 2018, 5

← 1 2 3 4 5 6 7 8 9 10 →