Structural studies of the enteroinvasive Escherichia coli (EIEC) O28 O-antigenic polysaccharide

被引:0
|
作者
Rundlof, T
Weintraub, A
Widmalm, G
机构
[1] UNIV STOCKHOLM,ARRHENIUS LAB,DEPT ORGAN CHEM,S-10691 STOCKHOLM,SWEDEN
[2] HUDDINGE UNIV HOSP,KAROLINSKA INST,DEPT IMMUNOL MICROBIOL PATHOL & INFECT DIS,DIV CLIN BACTERIOL,S-14186 HUDDINGE,SWEDEN
关键词
Escherichia coli; lipopolysaccharide; phosphodiester; teichoic acid type; enteroinvasive;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The structure of the O-specific side-chain of the lipopolysaccharide From Escherichia coli O28 has been investigated. NMR spectroscopy has been the main method used, complemented with sugar and methylation analyses, The polysaccharide contains one equivalent of O-acetyl groups per repeating unit. Selective cleavage of the O-deacetylated polymer was performed by treatment with aqueous hydrofluoric acid, and resulted in a trisaccharide-glycerol. The polysaccharide thus is of the teichoic acid type and composed of repeating units in which the trisaccharide-glycerol residues are joined by phosphodiester linkages. The O-antigen polysaccharide has the following structure. [GRAPHICS] The absolute configuration of the glycerol moiety as R, (i.e., D-glycerol 1-phosphate) was determined by a new method based on TEMPO oxidation of the polysaccharide, followed by GLC analysis of the (+)-2-butyl ester of the resulting glyceric acid. (C) 1996 Elsevier Science Ltd.
引用
收藏
页码:127 / 139
页数:13
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