Gold nanoparticle-based detection of genomic DNA targets on microarrays using a novel optical detection system

被引：216

作者：

Storhoff, JJ ^{[1
]}

Marla, SS ^{[1
]}

Bao, P ^{[1
]}

Hagenow, S ^{[1
]}

Mehta, H ^{[1
]}

Lucas, A ^{[1
]}

Garimella, V ^{[1
]}

Patno, T ^{[1
]}

Buckingham, W ^{[1
]}

Cork, W ^{[1
]}

Müller, UR ^{[1
]}

机构：

[1] Nanosphere Inc, Appl Res, Northbrook, IL 60062 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2004年 / 19卷 / 08期

关键词：

nanoparticles; DNA microarray; silver amplification; SNPs; Staphylococcus; thrombosis;

D O I：

10.1016/j.bios.2003.08.014

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The development of a nanoparticle-based detection methodology for sensitive and specific DNA-based diagnostic applications is described. The technology utilizes gold nanoparticles derivatized with thiol modified oligonucleotides that are designed to bind complementary DNA targets. A glass surface with arrays of immobilized oligonucleotide capture sequences is used to capture DNA targets, which are then detected via hybridization to the gold nanoparticle probes. Amplification with silver allows for detection and quantitation by measuring evanescent wave induced light scatter with low-cost optical detection systems. Compared to Cy3-based fluorescence, silver amplified gold nanoparticle probes provide for a similar to1000-fold increase in sensitivity. Furthermore, direct detection of non-amplified genomic DNA from infectious agents is afforded through increased specificity and even identification of single nucleotide polymorphisms (SNP) in human genomic DNA appears feasible. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：875 / 883

页数：9

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