Histone H2B Deacylation Selectivity: Exploring Chromatin's Dark Matter with an Engineered Sortase

被引：34

作者：

Wang, Zhipeng A. ^{[1
,2
]}

Whedon, Samuel D. ^{[1
,2
]}

Wu, Mingxuan ^{[1
,2
]}

Wang, Siyu ^{[3
]}

Brown, Edward A. ^{[3
]}

Anmangandla, Ananya ^{[4
]}

Regan, Liam ^{[3
]}

Lee, Kwangwoon ^{[1
,2
]}

Du, Jianfeng ^{[5
]}

Hong, Jun Young ^{[4
]}

Fairall, Louise ^{[3
]}

Kay, Taylor ^{[1
,2
]}

Lin, Hening ^{[4
]}

Zhao, Yingming ^{[5
]}

Schwabe, John W. R. ^{[3
]}

Cole, Philip A. ^{[1
,2
]}

机构：

[1] Brigham & Womens Hosp, Dept Med, Div Genet, Boston, MA 02115 USA

[2] Harvard Med Sch, Dept Biol Chem & Mol Pharmcol, Boston, MA 02115 USA

[3] Univ Leicester, Leicester Inst Struct & Chem Biol, Dept Mol & Cell Biol, Leicester LE1 7RH, Leics, England

[4] Cornell Univ, Howard Hughes Med Inst, Dept Chem & Chem Biol, Ithaca, NY 14853 USA

[5] Ben May Dept Canc Res, Chicago, IL 60637 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2022年 / 144卷 / 08期

基金：

英国惠康基金;

关键词：

METABOLIC-REGULATION; CHEMICAL-SYNTHESIS; GENE-EXPRESSION; DEACETYLASE; DEMETHYLATION; ACETYLATION; RECOGNITION; INHIBITORS; PROTEINS; BIOLOGY;

D O I：

10.1021/jacs.1c13555

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe a new method to produce histone H2B by semisynthesis with an engineered sortase transpeptidase. N-Terminal tail site-specifically modified acetylated, lactylated, and beta-hydroxybutyrylated histone H2Bs were incorporated into nucleosomes and investigated as substrates of histone deacetylase (HDAC) complexes and sirtuins. A wide range of rates and site-specificities were observed by these enzyme forms suggesting distinct biological roles in regulating chromatin structure and epigenetics.

引用

页码：3360 / 3364

页数：5

共 39 条

[1] Lysine Acetylation Goes Global: From Epigenetics to Metabolism and Therapeutics
Ali, Ibraheem
Conrad, Ryan J.
Verdin, Eric
Ott, Melanie
[J]. CHEMICAL REVIEWS, 2018, 118 (03) : 340 - 376
[2] Site-Specific Reactivity of Nonenzymatic Lysine Acetylation
Baeza, Josue
Smallegan, Michael J.
Denu, John M.
[J]. ACS CHEMICAL BIOLOGY, 2015, 10 (01) : 122 - 128
[3] Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes
Bantscheff, Marcus
Hopf, Carsten
Savitski, Mikhail M.
Dittmann, Antje
Grandi, Paola
Michon, Anne-Marie
Schlegl, Judith
Abraham, Yann
Becher, Isabelle
Bergamini, Giovanna
Boesche, Markus
Delling, Manja
Duempelfeld, Birgit
Eberhard, Dirk
Huthmacher, Carola
Mathieson, Toby
Poeckel, Daniel
Reader, Valerie
Strunk, Katja
Sweetman, Gavain
Kruse, Ulrich
Neubauer, Gitte
Ramsden, Nigel G.
Drewes, Gerard
[J]. NATURE BIOTECHNOLOGY, 2011, 29 (03) : 255 - U124
[4] Histone H4K20 Demethylation by Two hHR23 Proteins
Cao, Xiongwen
Chen, Yanran
Wu, Bin
Wang, Xiaoyun
Xue, Hongjuan
Yu, Lu
Li, Jie
Wang, Yiqin
Wang, Wei
Xu, Qing
Mao, Hailei
Peng, Chao
Han, Gang
Chen, Charlie Degui
[J]. CELL REPORTS, 2020, 30 (12): : 4152 - +
[5] Protein Lysine Acetylation by p300/CBP
Dancy, Beverley M.
Cole, Philip A.
[J]. CHEMICAL REVIEWS, 2015, 115 (06) : 2419 - 2452
[6] Differential HDAC1 and 2 Recruitment by Members of the MIER Family
Derwish, Roya
Paterno, Gary D.
Gillespie, Laura L.
[J]. PLOS ONE, 2017, 12 (01):
[7] Chemically Sumoylated Histone H4 Stimulates Intranucleosomal Demethylation by the LSD1-CoREST Complex
Dhall, Abhinav
Weller, Caroline E.
Chu, Aurea
Shelton, Patrick M. M.
Chatterjee, Champak
[J]. ACS CHEMICAL BIOLOGY, 2017, 12 (09) : 2275 - 2280
[8] Chromatin as a key consumer in the metabolite economy
Diehl, Katharine L.
Muir, Tom W.
[J]. NATURE CHEMICAL BIOLOGY, 2020, 16 (06) : 620 - 629
[9] Dyer PN, 2004, METHOD ENZYMOL, V375, P23
[10] Molecular recognition of the nucleosomal "supergroove"
Edayathumangalam, RS
Weyermann, P
Gottesfeld, JM
Dervan, PB
Luger, K
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (18) : 6864 - 6869

← 1 2 3 4 →