Novel IFNγ ELISPOT Assay for Detection of Functional Carcinoembryonic Antigen-Specific Chimeric Antigen Receptor-Redirected T Cells

被引：2
作者：
Elkord, E. [1 ,2 ]
Burt, D. J. [3 ]
机构：
[1] Univ Salford, Ctr Biochem Drug Design & Canc Res, Sch Environm & Life Sci, Salford M5 4WT, Lancs, England
[2] Univ Manchester, Dept Med Oncol, Sch Canc & Enabling Sci, Manchester Acad Hlth Sci Ctr, Manchester, Lancs, England
[3] Univ Manchester, Paterson Inst Canc Res, Clin Immune & Mol Monitoring Lab, Manchester, Lancs, England
来源：
SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 2011年 / 74卷 / 04期
关键词：
ADOPTIVE IMMUNOTHERAPY;
D O I：
10.1111/j.1365-3083.2011.02588.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFN gamma-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.
引用
收藏
页码：419 / 422
页数：4
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