Competitive immunoassay of phenobarbital by microchip electrophoresis with laser induced fluorescence detection

被引：14
作者：
Huang, Yong [1 ,2 ]
Zhao, Shulin [1 ]
Shi, Ming [1 ]
Liu, Jinwen [1 ]
Liang, Hong [1 ]
机构：
[1] Guangxi Normal Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Chem & Mol Engn Med Resources, Guilin 541004, Peoples R China
[2] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China
来源：
ANALYTICA CHIMICA ACTA | 2011年 / 694卷 / 1-2期
关键词：
Microchip electrophoresis; Competitive immunoassay; Laser induced fluorescence detection; Phenobarbital; Human plasma; SOLID-PHASE EXTRACTION; PERFORMANCE LIQUID-CHROMATOGRAPHY; TRANSFORM INFRARED-SPECTROSCOPY; CARBON-PASTE ELECTRODE; ANTIEPILEPTIC DRUGS; CAPILLARY-ELECTROPHORESIS; MASS-SPECTROMETRY; CHEMILUMINESCENCE DETECTION; HOMOGENEOUS IMMUNOASSAY; SERUM;
D O I：
10.1016/j.aca.2011.03.036
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A microchip electrophoresis method with laser induced fluorescence detection was developed for the immunoassay of phenobarbital. The detection was based on the competitive immunoreaction between analyte phenobarbital and fluorescein isothiocyanate (FITC) labeled phenobarbital with a limited amount of antibody. The assay was developed by varying the borate concentration, buffer pH, separation voltage, and incubation time. A running buffer system containing 35 mM borate and 15 mM sodium dodecyl sulfate (pH 9.5), and 2800 V separation voltage provided analysis conditions for a high-resolution, sensitive, and repeatable assay of phenobarbital. Free FITC-labeled phenobarbital and immunocomplex were separated within 30 s. The calibration curve for phenobarbital had a detection limit of 3.4 nM and a range of 8.6-860.0 nM. The assay could be used to determine the phenobarbital plasma concentration in clinical plasma sample. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：162 / 166
页数：5
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