Stable production of recombinant chicken antibody in CHO-K1 cell line

被引：15
作者：
Shimamoto, T
Nishibori, N
Aosasa, M
Horiuchi, H
Furusawa, S
Matsuda, H
机构：
[1] Hiroshima Univ, Grad Sch Biosphere Sci, Dept Mol & Appl Biosci, Immunobiol Lab, Higashihiroshima 7398528, Japan
[2] Hiroshima Prefectural Inst Ind Sci & Technol, Hiroshima 7390046, Japan
来源：
BIOLOGICALS | 2005年 / 33卷 / 03期
关键词：
recombinant antibody; chicken; IgY; CHO;
D O I：
10.1016/j.biologicals.2005.05.002
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
When compared with mammalian IgG, chicken IgY is advantageous in terms of cross-reactivity. In this study, two plasmids were constructed for expression of recombinant chicken IgY derived from a chicken hybridoma. The first was for expression of the light (L) chain, and the other was for the heavy (H) chain with a histidine (His) tag at the carboxy-terminal. After transfection of recombinant chicken IgY gene into Chinese hamster ovary cells, a transfectant designated HF33 that secreted the specific antibody was selected. HF33 cells produced recombinant IgY with His tag at 10-15 mu g/10(6) cells/24h. On Western blotting analysis, the recombinant IgY was detected as one band for the H chain and two bands for the L chain. The recombinant IgY was successfully purified in a one-step procedure using a nickel-affinity resin. These results indicate that the present recombinant chicken IgY is useful for further applications. (c) 2005 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：169 / 174
页数：6
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