Heterotrimeric Kinesin-2 (KIF3) Mediates Transition Zone and Axoneme Formation of Mouse Photoreceptors

被引：45

作者：

Jiang, Li ^{[1
]}

Wei, Yuxiao ^{[1
]}

Ronquillo, Cecinio C. ^{[1
]}

Marc, Robert E. ^{[1
]}

Yoder, Bradley K. ^{[3
]}

Frederick, Jeanne M. ^{[1
]}

Baehr, Wolfgang ^{[1
,2
,4
]}

机构：

[1] Univ Utah, Hlth Sci Ctr, Dept Ophthalmol & Visual Sci, Salt Lake City, UT 84132 USA

[2] Univ Utah, Hlth Sci Ctr, Dept Neurobiol & Anat, Salt Lake City, UT 84132 USA

[3] Univ Alabama Birmingham, Dept Cell Dev & Integrat Biol, Birmingham, AL 35294 USA

[4] Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2015年 / 290卷 / 20期

基金：

美国国家卫生研究院;

关键词：

POLYCYSTIC KIDNEY-DISEASE; INTRAFLAGELLAR TRANSPORT; PRIMARY CILIUM; OUTER SEGMENT; IFT-PARTICLES; SENSORY CILIA; GENE TG737; PROTEIN; ROD; TRAFFICKING;

D O I：

10.1074/jbc.M115.638437

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anterograde intraflagellar transport (IFT) employing kinesin-2 molecular motors has been implicated in trafficking of photoreceptor outer segment proteins. We generated embryonic retina-specific (prefix "emb") and adult tamoxifen-induced (prefix "tam") deletions of KIF3a and IFT88 in adult mice to study photoreceptor ciliogenesis and protein trafficking. In (emb)Kif3a(-/-) and in (emb)Ift88(-/-) mice, basal bodies failed to extend transition zones (connecting cilia) with outer segments, and visual pigments mistrafficked. In contrast, (tam)Kif3a(-/-) and (tam)Ift88(-/-) photoreceptor axonemes disintegrated slowly post-induction, starting distally, but rhodopsin and cone pigments trafficked normally for more than 2 weeks, a time interval during which the outer segment is completely renewed. The results demonstrate that visual pigments transport to the retinal outer segment despite removal of KIF3 and IFT88, and KIF3-mediated anterograde IFT is responsible for photoreceptor transition zone and axoneme formation.

引用

页码：12765 / 12778

页数：14

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