Dectin-1 mediates in vitro phagocytosis of Candida albicans yeast cells by retinal microglia

被引：34
|
作者：
Maneu, Victoria [2 ,3 ]
Yanez, Alberto [1 ]
Murciano, Celia [1 ]
Molina, Andres [1 ]
Luisa Gil, Maria [1 ]
Gozalbo, Daniel [1 ]
机构：
[1] Univ Valencia, Dept Microbiol & Ecol, Fac Farm, E-46100 Burjassot, Spain
[2] Univ Alicante, Dept Opt Farmacol & Anat, San Vicente Del Raspeig, Spain
[3] Univ Autonoma Madrid, Inst Teofilo Hernando I D Medicamento, Madrid, Spain
来源：
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2011年 / 63卷 / 01期
关键词：
Candida albicans; retinal microglia; Dectin-1; TLR2; phagocytosis; flow cytometry; CYTOKINE PRODUCTION; INFECTIONS; RECEPTORS;
D O I：
10.1111/j.1574-695X.2011.00829.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We have investigated the expression of TLR2 and Dectin-1 in retinal microglia and their involvement in Candida albicans phagocytosis using a cytometric approach. The expression of both receptors has been demonstrated in CD11b(+) retinal cells. Phagocytosis of pHrodo-labelled C. albicans yeasts by microglial CD11b(+) cells of C57BL/6 mice was inhibited both by the Dectin-1 antagonist laminarin and anti-Dectin-1 antibodies, whereas phagocytosis of yeasts by retinal microglia of TLR2 KO mice was unaffected. These data indicate that phagocytosis of C. albicans yeasts by retinal microglia is mediated by Dectin-1, whereas TLR2 does not play a significant role in this process.
引用
收藏
页码：148 / 150
页数：3
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