PREPARATIVE ISOLATION OF STEROIDAL SAPONINS FROM GARLIC (ALLIUM SATIVUM L.) USING HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY COUPLED WITH EVAPORATIVE LIGHT SCATTERING DETECTION

被引：4

作者：

Ma, Qiang ^{[1
]}

Luo, Jianguang ^{[1
]}

Kong, Lingyi ^{[1
]}

机构：

[1] China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Jiangsu, Peoples R China

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2011年 / 34卷 / 17期

关键词：

Allium sativum L; buffer salt; emulsification; garlic; HSCCC; steroidal saponins; SUPERCRITICAL-FLUID EXTRACTION; PURIFICATION; SEPARATION; IDENTIFICATION; CHEMISTRY; BULBS; ROOT;

D O I：

10.1080/10826076.2011.582911

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was successfully applied for preparative separation and purification of four steroidal saponins (compounds 1-4) from crude extracts of garlic (Allium sativum L.) by one-step separation, using n-butanol-acetic acid-6 mM aqueous ammonium acetate (4: 1: 5, v/v/v) as a solvent system. The quantities of compounds 1-4 isolated from 300 mg of the crude sample were 23.5 mg, 14.7 mg, 18.2 mg, and 7.0 mg, respectively. Their purities were found to be over 93.0% by HPLC-ELSD analysis. Their chemical structures were confirmed by ESI-MS, H-1-and C-13-NMR. This is the first time steroidal saponins have been separated using HSCCC-ELSD.

引用

页码：1997 / 2007

页数：11

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