Study of the Alkyl Chain Length on Laccase Stability and Enzymatic Kinetic with Imidazolium Ionic Liquids

被引：37
|
作者：
Rodriguez, Oscar [1 ]
Cristovao, Raquel O. [1 ]
Tavares, Ana P. M. [1 ]
Macedo, Eugenia A. [1 ]
机构：
[1] Univ Porto, LSRE, Associate Lab LSRE LCM, Dept Chem Engn,Fac Engn, P-4200465 Oporto, Portugal
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2011年 / 164卷 / 04期
关键词：
Ionic liquids; Laccase; Stability; Activity; Kinetic parameters; REACTIVE TEXTILE DYES; CERRENA-UNICOLOR; FUNGAL LACCASES; ORGANIC MEDIA; OXIDATION; HYDROLYSIS; LIPASE; WATER; DECOLORIZATION; DEGRADATION;
D O I：
10.1007/s12010-010-9154-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The activity and stability of laccase and their kinetic mechanisms in water soluble ionic liquids (ILs): 1-butyl-3-methyl imidazolium chloride [C(4)mim][Cl], 1-octyl-3-methyl imidazolium chloride [C(8)mim][Cl], and 1-decyl-3-methyl imidazolium chloride [C(10)mim][Cl] were investigated. The results show that an IL concentration up to 10% is satisfactory for initial laccase activity at pH 9.0. The laccase stability was well maintained in [C(4)mim][Cl] IL when compared to the control. The inactivation of laccase increases with the length of the alkyl chain in the IL: [C(10)mim][Cl] > [C(8)mim][Cl] > [C(4)mim][Cl]. The kinetic studies in the presence of ABTS as substrate allowed calculating the Michaelis-Menten parameters. Among the ILs, [C(4)mim][Cl] was the suitable choice attending to laccase activity and stability. Alkyl chains in the ions of ILs have a deactivating effect on laccase, which increases strongly with the length of the alkyl chain.
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页码：524 / 533
页数：10
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