Study of the Alkyl Chain Length on Laccase Stability and Enzymatic Kinetic with Imidazolium Ionic Liquids

被引：37
|
作者：
Rodriguez, Oscar [1 ]
Cristovao, Raquel O. [1 ]
Tavares, Ana P. M. [1 ]
Macedo, Eugenia A. [1 ]
机构：
[1] Univ Porto, LSRE, Associate Lab LSRE LCM, Dept Chem Engn,Fac Engn, P-4200465 Oporto, Portugal
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2011年 / 164卷 / 04期
关键词：
Ionic liquids; Laccase; Stability; Activity; Kinetic parameters; REACTIVE TEXTILE DYES; CERRENA-UNICOLOR; FUNGAL LACCASES; ORGANIC MEDIA; OXIDATION; HYDROLYSIS; LIPASE; WATER; DECOLORIZATION; DEGRADATION;
D O I：
10.1007/s12010-010-9154-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The activity and stability of laccase and their kinetic mechanisms in water soluble ionic liquids (ILs): 1-butyl-3-methyl imidazolium chloride [C(4)mim][Cl], 1-octyl-3-methyl imidazolium chloride [C(8)mim][Cl], and 1-decyl-3-methyl imidazolium chloride [C(10)mim][Cl] were investigated. The results show that an IL concentration up to 10% is satisfactory for initial laccase activity at pH 9.0. The laccase stability was well maintained in [C(4)mim][Cl] IL when compared to the control. The inactivation of laccase increases with the length of the alkyl chain in the IL: [C(10)mim][Cl] > [C(8)mim][Cl] > [C(4)mim][Cl]. The kinetic studies in the presence of ABTS as substrate allowed calculating the Michaelis-Menten parameters. Among the ILs, [C(4)mim][Cl] was the suitable choice attending to laccase activity and stability. Alkyl chains in the ions of ILs have a deactivating effect on laccase, which increases strongly with the length of the alkyl chain.
引用
收藏
页码：524 / 533
页数：10
相关论文
共 50 条
  • [1] Study of the Alkyl Chain Length on Laccase Stability and Enzymatic Kinetic with Imidazolium Ionic Liquids
    Oscar Rodríguez
    Raquel O. Cristóvão
    Ana P. M. Tavares
    Eugénia A. Macedo
    Applied Biochemistry and Biotechnology, 2011, 164 : 524 - 533
  • [2] Vaporization Enthalpies of Imidazolium Based Ionic Liquids: Dependence on Alkyl Chain Length
    Zaitsau, Dzmitry H.
    Verevkin, Sergey P.
    Emel'yanenko, Vladimir N.
    Heintz, Andreas
    CHEMPHYSCHEM, 2011, 12 (18) : 3609 - 3613
  • [3] Effect of alkyl chain length on the wetting behavior of imidazolium based ionic liquids: A molecular dynamics study
    Bhattacharjee, Sanchari
    Khan, Sandip
    FLUID PHASE EQUILIBRIA, 2019, 501
  • [4] Uptake and accumulation of imidazolium ionic liquids in rice seedlings: Impacts of alkyl chain length
    Habibul, Nuzahat
    Ilmurat, Mihriban
    Habibul, Zumrat
    Hu, Yi
    Ma, Xiaoli
    CHEMOSPHERE, 2020, 242
  • [5] Vaporization enthalpies of imidazolium based ionic liquids. A thermogravimetric study of the alkyl chain length dependence
    Verevkin, Sergey P.
    Zaitsau, Dzmitry H.
    Emel'yanenko, Vladimir N.
    Ralys, Ricardas V.
    Yermalayeu, Andrei V.
    Schick, Christoph
    JOURNAL OF CHEMICAL THERMODYNAMICS, 2012, 54 : 433 - 437
  • [6] Effect of alkyl chain length on adsorption properties of alkyl imidazolium ionic liquids surface imprinting polymers
    Gao, Xia
    Li, Rui
    Zhu, Guifen
    Fan, Jing
    MONATSHEFTE FUR CHEMIE, 2015, 146 (03): : 475 - 484
  • [7] Effect of alkyl chain length on adsorption properties of alkyl imidazolium ionic liquids surface imprinting polymers
    Xia Gao
    Rui Li
    Guifen Zhu
    Jing Fan
    Monatshefte für Chemie - Chemical Monthly, 2015, 146 : 475 - 484
  • [8] Imidazolium Based Ionic Liquids: Impact of the Cation Symmetry and Alkyl Chain Length on the Enthalpy of Vaporization
    Yermalayeu, Andrei V.
    Zaitsau, Dzmitry H.
    Loor, Manuel
    Schaumann, Julian
    Emel'yanenko, Vladimir N.
    Schulz, Stephan
    Verevkin, Sergey P.
    ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE, 2017, 643 (01): : 81 - 86
  • [9] Probing the impact of alkyl chain length of imidazolium ionic liquids on the conformational stability of collagen type-I from skin of Lutjanus erythropterus
    Ahmed, Mofieed
    Verma, Amit Kumar
    Malik, Maqsood Ahmad
    Alzahrani, Khalid Ahmed
    Patel, Rajan
    JOURNAL OF MOLECULAR STRUCTURE, 2023, 1290
  • [10] Alkyl-chain length effects of imidazolium and pyridinium ionic liquids on photosynthetic response of Pseudokirchneriella subcapitata
    Pham, Thi Phuong Thuy
    Cho, Chul-Woong
    Min, Jiho
    Yun, Yeoung-Sang
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2008, 105 (04) : 425 - 428
12345