Technical aspects of immunohistochemistry

被引：514
|
作者：
Ramos-Vara, JA
机构：
[1] Purdue Univ, Sch Vet Med, Anim Dis Diagnost Lab, W Lafayette, IN 47907 USA
[2] Purdue Univ, Sch Vet Med, Dept Vet Pathobiol, W Lafayette, IN 47907 USA
来源：
VETERINARY PATHOLOGY | 2005年 / 42卷 / 04期
关键词：
antibodies; antigen retrieval; antigens; detection methods; fixation; immunohistochemistry;
D O I：
10.1354/vp.42-4-405
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Immunohistochemistry is an integral technique in many veterinary laboratories for diagnostic and research purposes. In the last decade, the ability to detect antigens (Ags) in tissue sections has improved dramatically, mainly by countering the deleterious effects of formaldehyde with antigen retrieval (AR) and increasing sensitivity of the detection systems. In this review, I address these topics and provide an overview of technical aspects of immuuohistochemistry, including those related to antibodies (Abs) and Ags, fixation, AR, detection methods, background, and troubleshooting. Microarray technology and the use of rabbit monoclonal Abs in immunohistochemistry are also discussed.
引用
收藏
页码：405 / 426
页数：22
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