Comprehensive characterization of surface-functionalized poly (amidoamine) dendrimers with acetamide, hydroxyl, and carboxyl groups

被引:74
作者
Shi, XY [1 ]
Lesniak, W [1 ]
Islam, MT [1 ]
Muñiz, MC [1 ]
Balogh, LP [1 ]
Baker, JR [1 ]
机构
[1] Univ Michigan, Dept Internal Med, Ctr Biol Nanotechnol, Ann Arbor, MI 48109 USA
关键词
dendrimers; functionalization of polymers; synthesis; mass spectrometry; electrophoresis;
D O I
10.1016/j.colsurfa.2005.07.031
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Terminal amine groups of poly(amidoamine) (PAMAM) dendrimers can be substituted with different functional groups for various applications. In this study, PAMAM derivatives with acetamide, hydroxyl, and carboxyl termini were synthesized from ethylenediamine (EDA) core generation 4 and 5 primary amine-terminated PAMAM dendrimers. The reaction products were purified with dialysis and subsequently characterized by polyacrylamide gel electrophoresis (PAGE), capillary electrophoresis (CE), size exclusion chromatography (SEC), matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, potentiometric titration, H-1 NMR, and C-13 NMR. PAGE and CE electropherograms provide data regarding the purity, charge distribution, and electrophoretic mobility of the dendrimers and their derivatives. SEC and MALDI-TOF mass spectrometry detect the average absolute molar mass and the individual mass fractions, respectively. The combination of SEC with potentiometric titration provides quantitative evidence of the degree of the functional group substitution, while NMR techniques (both H-1 NMR and C-13 NMR) confirmed the changes in dendrimer surface functionalization. This study provides a general example for the comprehensive characterization of surface-functionalized PAMAM dendrimer nanoparticles. The synthesized dendrimer derivatives hold promise for environmental and medical applications. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:139 / 150
页数:12
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