Generation of pluripotent stem cells from adult mouse liver and stomach cells

被引：728

作者：

Aoi, Takashi ^{[1
,2
]}

Yae, Kojiro ^{[1
]}

Nakagawa, Masato ^{[1
]}

Ichisaka, Tomoko ^{[1
,3
]}

Okita, Keisuke ^{[1
]}

Takahashi, Kazutoshi ^{[1
]}

Chiba, Tsutomu ^{[2
]}

Yamanaka, Shinya ^{[1
,3
,4
,5
]}

机构：

[1] Kyoto Univ, Dept Stem Cell Biol, Inst Frontier Med Sci, Kyoto 6068507, Japan

[2] Kyoto Univ, Dept Gastroenterol & Hepatol, Grad Sch Med, Kyoto 6068507, Japan

[3] Japan Sci & Technol Agcy, CREST, Kawaguchi, Saitama 3320012, Japan

[4] Gladstone Inst Cardiovasc Dis, San Francisco, CA 94158 USA

[5] Kyoto Univ, Ctr Induced Pluripotent StemiPS Cell Res & Applic, Inst Integrated Cell Mat Sci, Kyoto 6068507, Japan

来源：

SCIENCE | 2008年 / 321卷 / 5889期

关键词：

D O I：

10.1126/science.1154884

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Induced pluripotent stem ( iPS) cells have been generated from mouse and human fibroblasts by the retroviral transduction of four transcription factors. However, the cell origins and molecular mechanisms of iPS cell induction remain elusive. This report describes the generation of iPS cells from adult mouse hepatocytes and gastric epithelial cells. These iPS cell clones appear to be equivalent to embryonic stem cells in gene expression and are competent to generate germline chimeras. Genetic lineage tracings show that liver- derived iPS cells are derived from albumin-expressing cells. No common retroviral integration sites are found among multiple clones. These data suggest that iPS cells are generated by direct reprogramming of lineage- committed somatic cells and that retroviral integration into specific sites is not required.

引用

页码：699 / 702

页数：4

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