Rapid method for evaluating reversed-phase high-performance liquid chromatography column stability

被引：28
作者：
Fonseca, DA
Gutiérrez, HR
Collins, KE
Collins, CH
机构：
[1] Univ Estadual Campinas, Inst Quim, BR-13084971 Campinas, SP, Brazil
[2] Univ Estadual Campinas, Inst Fis, BR-13084971 Campinas, SP, Brazil
来源：
JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1030卷 / 1-2期
关键词：
stationary phases; LC; stability studies;
D O I：
10.1016/j.chroma.2003.12.033
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A procedure is presented for the rapid evaluation of HPLC stationary phase stability at pH 8.4 or 10.1 using a temperature of 60degreesC. Mobile phase (MeOH-0.1 mol 1(-1) aqueous NaHCO3, 50:50, v/v) is continuously passed through the column with periodic injections of a test solution until the several chromatographic parameters of the resulting chromatograms are degraded. The tests were applied to several commercial and laboratory-made stationary phases. After degradation two of these phases, one commercial and one laboratory-made, were examined by elemental analysis and scanning electron microscopy to elucidate the degradation process. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：149 / 155
页数：7
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