Sentinel lymph node biopsy with an indocyanine green-based near-infrared fluorescence imaging system avoids the shortcomings of using a radioisotope or a combination of a blue dye and a radioactive tracer. To improve surgical precision, recent research has provided a depth profile of the sentinel lymph node by fusing fluorescence and ultrasound imaging. Here, we present a combined near-infrared fluorescence and ultrasound imaging system based on a transparent ultrasound transducer. The transparent ultrasound transducer enables seamless coaxial alignment of the fluorescence and ultrasound beam paths, allowing bi-modal observation of a single region of interest. Further, we demonstrate that the sentinel lymph node of mice injected with indocyanine green can be successfully localized and dissected based on information from the bi-modal imaging system. (C) 2022 Optica Publishing Group
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Beth Israel Deaconess Med Ctr, Dept Med, Div Hematol Oncol, Boston, MA 02215 USALeiden Univ, Med Ctr, Dept Surg, NL-2300 RC Leiden, Netherlands
Moffitt, Lorissa A.
Rosenberg, Mireille
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Beth Israel Deaconess Med Ctr, Dept Med, Div Hematol Oncol, Boston, MA 02215 USALeiden Univ, Med Ctr, Dept Surg, NL-2300 RC Leiden, Netherlands
Rosenberg, Mireille
Hirshfield-Bartek, Judith
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Dana Farber Brigham & Womens Canc Ctr, Dept Surg, Boston, MA USALeiden Univ, Med Ctr, Dept Surg, NL-2300 RC Leiden, Netherlands
Hirshfield-Bartek, Judith
Gioux, Sylvain
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Beth Israel Deaconess Med Ctr, Dept Med, Div Hematol Oncol, Boston, MA 02215 USALeiden Univ, Med Ctr, Dept Surg, NL-2300 RC Leiden, Netherlands
Gioux, Sylvain
van de Velde, Cornelis J. H.
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Leiden Univ, Med Ctr, Dept Surg, NL-2300 RC Leiden, NetherlandsLeiden Univ, Med Ctr, Dept Surg, NL-2300 RC Leiden, Netherlands
van de Velde, Cornelis J. H.
Vahrmeijer, Alexander L.
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Leiden Univ, Med Ctr, Dept Surg, NL-2300 RC Leiden, NetherlandsLeiden Univ, Med Ctr, Dept Surg, NL-2300 RC Leiden, Netherlands
Vahrmeijer, Alexander L.
Frangioni, John V.
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Beth Israel Deaconess Med Ctr, Dept Med, Div Hematol Oncol, Boston, MA 02215 USA
Beth Israel Deaconess Med Ctr, Dept Radiol, Boston, MA 02215 USALeiden Univ, Med Ctr, Dept Surg, NL-2300 RC Leiden, Netherlands