Knockdown of Lrp1 in RAW264 cells inhibits osteoclast differentiation and osteoclast-osteoblast interactions in vitro

被引：14

作者：

Kohara, Yukihiro ^{[1
]}

Haraguchi, Ryuma ^{[1
]}

Kitazawa, Riko ^{[1
,2
]}

Kitazawa, Sohei ^{[1
]}

机构：

[1] Ehime Univ, Dept Mol Pathol, Grad Sch Med, Toon City, Ehime 7910295, Japan

[2] Ehime Univ Hosp, Div Diagnost Pathol, Toon City, Ehime 7910295, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 523卷 / 04期

关键词：

RAW264; cells; Macrophages; Osteoclasts; LRP1; CD91; Osteoclast-osteoblast interactions; BONE-FORMATION; VI COLLAGEN; ASSOCIATION; ACTIVATION; NFATC1; MICE;

D O I：

10.1016/j.bbrc.2020.01.065

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Low density lipoprotein receptor-related protein 1 (LRP1), a multifunctional cell surface protein, is expressed in bone marrow-derived macrophages. While LRP1 is thought to be a suppressor of osteoclast differentiation at late stages, its function at early stages remains unclear. Here we demonstrate that Lrp1 stable knockdown by lentiviral short hairpin RNA in macrophage cell line RAW264 cells inhibited RANKL-induced osteoclast formation and osteoclastic master transcription factor Nfatc1 mRNA expression as assessed by quantitative RT-PCR. Furthermore, knockdown of the Lrp1 gene suppressed not only differentiation, but also proliferation, and inhibitory effects on osteoblastic ALP activity by osteoclast-derived humoral factors. Thus, we propose that LRP1 in macrophages is required for both differentiation into osteoclasts and osteoclast-osteoblast interactions. (C) 2020 Elsevier Inc. All rights reserved.

引用

页码：961 / 965

页数：5

共 25 条

[1] NFATc1 in mice represses osteoprotegerin during osteoclastogenesis and dissociates systemic osteopenia from inflammation in cherubism [J].

Aliprantis, Antonios O. ;

Ueki, Yasuyoshi ;

Sulyanto, Rosalyn ;

Park, Arnold ;

Sigrist, Kirsten S. ;

Sharma, Sudarshana M. ;

Ostrowski, Michael C. ;

Olsen, Bjorn R. ;

Glimcher, Laurie H. .

JOURNAL OF CLINICAL INVESTIGATION, 2008, 118 (11) :3775-3789

[2] Lrp1 in osteoblasts controls osteoclast activity and protects against osteoporosis by limiting PDGF-RANKL signaling [J].

Bartelt, Alexander ;

Behler-Janbeck, Friederike ;

Beil, F. Timo ;

Koehne, Till ;

Mueller, Brigitte ;

Schmidt, Tobias ;

Heine, Markus ;

Ochs, Laura ;

Yilmaz, Tayfun ;

Dietrich, Martin ;

Tuckermann, Jan P. ;

Amling, Michael ;

Herz, Joachim ;

Schinke, Thorsten ;

Heeren, Joerg ;

Niemeier, Andreas .

BONE RESEARCH, 2018, 6

[3] Activated α2-macroglobulin induces cell proliferation and mitogen-activated protein kinase activation by LRP-1 in the J774 macrophage-derived cell line [J].

Bonacci, Gustavo R. ;

Caceres, Leandro C. ;

Sanchez, Maria C. ;

Chiabrando, Gustavo A. .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2007, 460 (01) :100-106

[4] Low Density Receptor-Related Protein 1 Interactions With the Extracellular Matrix: More Than Meets the Eye [J].

Bres, Ewa E. ;

Faissner, Andreas .

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2019, 7

[5] PLEKHM1/DEF8/RAB7 complex regulates lysosome positioning and bone homeostasis [J].

Fujiwara, Toshifumi ;

Ye, Shiqiao ;

Castro-Gomes, Thiago ;

Winchell, Caylin G. ;

Andrews, Norma W. ;

Voth, Daniel E. ;

Varughese, Kottayil I. ;

Mackintosh, Samuel G. ;

Feng, Yunfeng ;

Pavlos, Nathan ;

Nakamura, Takashi ;

Manolagas, Stavros C. ;

Zhao, Haibo .

JCI INSIGHT, 2016, 1 (17)

[6] Lkb1 deletion in periosteal mesenchymal progenitors induces osteogenic tumors through mTORC1 activation [J].

Han, Yujiao ;

Feng, Heng ;

Sun, Jun ;

Liang, Xiaoting ;

Wang, Zhuo ;

Xing, Wenhui ;

Dai, Qinggang ;

Yang, Yang ;

Han, Anjia ;

Wei, Zhanying ;

Bi, Qing ;

Ji, Hongbin ;

Kang, Tiebang ;

Zou, Weiguo .

JOURNAL OF CLINICAL INVESTIGATION, 2019, 129 (05) :1895-1909

[7] Glucose metabolism in bone [J].

Karner, Courtney M. ;

Long, Fanxin .

BONE, 2018, 115 :2-7

[8] Distribution of type VI collagen in association with osteoblast lineages in the groove of Ranvier during rat postnatal development [J].

Kohara, Yukihiro ;

Soeta, Satoshi ;

Izu, Yayoi ;

Arai, Kiyotaka ;

Amasaki, Hajime .

ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER, 2016, 208 :58-68

[9] Accumulation of type VI collagen in the primary osteon of the rat femur during postnatal development [J].

Kohara, Yukihiro ;

Soeta, Satoshi ;

Izu, Yayoi ;

Amasaki, Hajime .

JOURNAL OF ANATOMY, 2015, 226 (05) :478-488

[10] Osteoclast-derived exosomal miR-214-3p inhibits osteoblastic bone formation [J].

Li, Defang ;

Liu, Jin ;

Guo, Baosheng ;

Liang, Chao ;

Dang, Lei ;

Lu, Cheng ;

He, Xiaojuan ;

Cheung, Hilda Yeuk-Siu ;

Xu, Liang ;

Lu, Changwei ;

He, Bing ;

Liu, Biao ;

Shaikh, Atik Badshah ;

Li, Fangfei ;

Wang, Luyao ;

Yang, Zhijun ;

Au, Doris Wai-Ting ;

Peng, Songlin ;

Zhang, Zongkang ;

Zhang, Bao-Ting ;

Pan, Xiaohua ;

Qian, Airong ;

Shang, Peng ;

Xiao, Lianbo ;

Jiang, Baohong ;

Wong, Chris Kong-Chu ;

Xu, Jiake ;

Bian, Zhaoxiang ;

Liang, Zicai ;

Guo, De-an ;

Zhu, Hailong ;

Tan, Weihong ;

Lu, Aiping ;

Zhang, Ge .

NATURE COMMUNICATIONS, 2016, 7

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