Expanding the range of substrate acceptance of enzymes: Combinatorial active-site saturation test

被引：364

作者：

Reetz, MT ^{[1
]}

Bocola, M ^{[1
]}

Carballeira, JD ^{[1
]}

Zha, DX ^{[1
]}

Vogel, A ^{[1
]}

机构：

[1] Max Planck Inst Kohlenforsch, D-45470 Mulheim, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 27期

关键词：

directed evolution; enzymes; lipases; protein engineering; substrate acceptance;

D O I：

10.1002/anie.200500767

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) CASTing for success: The traditional problem of expanding the range of substrate acceptance of enzymes can be solved by creating focused libraries of mutants resulting from randomization of pairs of properly chosen amino acids around the active site (see example with the lipase from Pseudomonas aeruginosa, amino acid pairs are shown in the same color). CAST = combinatorial active-site saturation test. © 2005 Wiley-VCH Verlag GmbH &, Co. KGaA.

引用

页码：4192 / 4196

页数：5

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