Endothelial cell culture:: protocol to obtain and cultivate human umbilical endothelial cells

被引：172

作者：

Marin, V ^{[1
]}

Kaplanski, G ^{[1
]}

Grès, S ^{[1
]}

Farnarier, C ^{[1
]}

Bongrand, P ^{[1
]}

机构：

[1] Hop St Marguerite, Immunol Lab, INSERM U387, F-13009 Marseille, France

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2001年 / 254卷 / 1-2期

关键词：

endothelial cell; umbilical vein; adhesion molecules; inflammation;

D O I：

10.1016/S0022-1759(01)00408-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Endothelial cells play a key role in prominent immunological and pathological processes such as leukocyte trafficking, inflammation, atheroma or cancer cell metastasis. Umbilical veins are probably the most widely used source for human endothelial cells, since they are more easily available than many other vessels, they are free from any pathological process and they are physiologically more relevant than many established cell lines. Here, we describe a standard protocol for preparation, maintenance and quality control of these cells. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：183 / 190

页数：8

共 35 条

[1] Each step during transendothelial migration of flowing neutrophils is regulated by the stimulatory concentration of tumour necrosis factor-α [J].

Bahra, P ;

Rainger, GE ;

Wautier, JL ;

Luu, NT ;

Nash, GB .

CELL ADHESION AND COMMUNICATION, 1998, 6 (06) :491-501

[2] GROWTH-CHARACTERISTICS OF CULTURED HUMAN MACROVASCULAR VENOUS AND ARTERIAL AND MICROVASCULAR ENDOTHELIAL-CELLS [J].

BEEKHUIZEN, H ;

VANFURTH, R .

JOURNAL OF VASCULAR RESEARCH, 1994, 31 (04) :230-239

[3]

BEVILACQUA MP, 1993, ANNU REV IMMUNOL, V11, P767, DOI 10.1146/annurev.iy.11.040193.004003

[4] SPECIAL REPORT - THE COMPLEXITY OF ENDOTHELIAL-CELLS [J].

FAJARDO, LF .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1989, 92 (02) :241-250

[5]

GIBOFSKY A, 1975, J IMMUNOL, V115, P730

[6]

Gillis C, 1995, CELL MOL BIOL, V41, P1131

[7] Modulation of human endothelial coil proliferation and migration by fucoidan and heparin [J].

Giraux, JL ;

Matou, S ;

Bros, A ;

Tapon-Bretaudière, J ;

Letourneur, D ;

Fischer, AM .

EUROPEAN JOURNAL OF CELL BIOLOGY, 1998, 77 (04) :352-359

[8] Growth requirements of endothelial cells in culture: Variations in serum and amino acid concentrations [J].

Gorman, L ;

Mercer, LP ;

Hennig, B .

NUTRITION, 1996, 12 (04) :266-270

[9] CONTROL OF PROLIFERATION OF HUMAN VASCULAR ENDOTHELIAL CELLS - CHARACTERIZATION OF RESPONSE OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS TO FIBROBLAST GROWTH-FACTOR, EPIDERMAL GROWTH-FACTOR, AND THROMBIN [J].

GOSPODAROWICZ, D ;

BROWN, KD ;

BIRDWELL, CR ;

ZETTER, BR .

JOURNAL OF CELL BIOLOGY, 1978, 77 (03) :774-788

[10] HEPARIN PROTECTS BASIC AND ACIDIC FGF FROM INACTIVATION [J].

GOSPODAROWICZ, D ;

CHENG, J .

JOURNAL OF CELLULAR PHYSIOLOGY, 1986, 128 (03) :475-484

← 1 2 3 4 →