A transmembrane form of annexin XII detected by site-directed spin labeling

被引：89
作者：
Langen, R
Isas, JM
Hubbell, WL
Haigler, HT [1 ]
机构：
[1] Univ Calif Irvine, Dept Physiol & Biophys, Irvine, CA 92697 USA
[2] Univ Calif Los Angeles, Jules Stein Eye Inst, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 24期
关键词：
D O I：
10.1073/pnas.95.24.14060
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Previous studies of the annexin family of Ca2+ binding proteins identified a soluble monomer in the absence of Ca2+ and a trimer adsorbed on the membrane surface in the presence of Ca2+. On the basis of site-directed spin-labeling studies of annexin XII at low pH, we now report a membrane-inserted form of the protein with a dramatically different structure. The data suggest that upon insertion a continuous transmembrane alpha-helix is reversibly formed from a helix-loop-helix motif in the solution structure. Other regions with similar membrane-insertion potential were identified in the amino acid sequence, and we propose that the corresponding helices come together to form an aqueous pore that mediates the ion channel activity reported for several annexins.
引用
收藏
页码：14060 / 14065
页数：6
相关论文
共 25 条
[1]   Structural features and light-dependent changes in the cytoplasmic interhelical E-F loop region of rhodopsin: A site-directed spin-labeling study [J].
Altenbach, C ;
Yang, K ;
Farrens, DL ;
Farahbakhsh, ZT ;
Khorana, HG ;
Hubbell, WL .
BIOCHEMISTRY, 1996, 35 (38) :12470-12478
[2]   A COLLISION GRADIENT-METHOD TO DETERMINE THE IMMERSION DEPTH OF NITROXIDES IN LIPID BILAYERS - APPLICATION TO SPIN-LABELED MUTANTS OF BACTERIORHODOPSIN [J].
ALTENBACH, C ;
GREENHALGH, DA ;
KHORANA, HG ;
HUBBELL, WL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (05) :1667-1671
[3]   TRANSMEMBRANE PROTEIN-STRUCTURE - SPIN LABELING OF BACTERIORHODOPSIN MUTANTS [J].
ALTENBACH, C ;
MARTI, T ;
KHORANA, HG ;
HUBBELL, WL .
SCIENCE, 1990, 248 (4959) :1088-1092
[4]   STRUCTURE OF INFLUENZA HEMAGGLUTININ AT THE PH OF MEMBRANE-FUSION [J].
BULLOUGH, PA ;
HUGHSON, FM ;
SKEHEL, JJ ;
WILEY, DC .
NATURE, 1994, 371 (6492) :37-43
[5]   Influenza hemagglutinin is spring-loaded by a metastable native conformation [J].
Carr, CM ;
Chaudhry, C ;
Kim, PS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (26) :14306-14313
[6]   A SPRING-LOADED MECHANISM FOR THE CONFORMATIONAL CHANGE OF INFLUENZA HEMAGGLUTININ [J].
CARR, CM ;
KIM, PS .
CELL, 1993, 73 (04) :823-832
[7]   ANNEXIN-V - THE KEY TO UNDERSTANDING ION SELECTIVITY AND VOLTAGE REGULATION [J].
DEMANGE, P ;
VOGES, D ;
BENZ, J ;
LIEMANN, S ;
GOTTIG, P ;
BERENDES, R ;
BURGER, A ;
HUBER, R .
TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (07) :272-276
[8]   Requirement of rigid-body motion of transmembrane helices for light activation of rhodopsin [J].
Farrens, DL ;
Altenbach, C ;
Yang, K ;
Hubbell, WL ;
Khorana, HG .
SCIENCE, 1996, 274 (5288) :768-770
[9]   CONTINUOUS AND STOPPED FLOW ELECTRON-PARAMAGNETIC-RES SPECTROMETER BASED ON A LOOP GAP RESONATOR [J].
HUBBELL, WL ;
FRONCISZ, W ;
HYDE, JS .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1987, 58 (10) :1879-1886
[10]   Watching proteins move using site-directed spin labeling [J].
Hubbell, WL ;
Mchaourab, HS ;
Altenbach, C ;
Lietzow, MA .
STRUCTURE, 1996, 4 (07) :779-783
123