Enhanced intracellular Ca2+ concentrations in Escherichia coli and Bacillus subtilis after addition of oligosaccharide elicitors

被引：10

作者：

Murphy, Tania M. ^{[1
]}

Nilsson, Alex Y. ^{[2
]}

Roy, Ipsita ^{[1
]}

Harrop, Anthony ^{[3
]}

Dixon, Keith ^{[3
]}

Keshavarz, Tajalli ^{[1
]}

机构：

[1] Univ Westminster, Appl Biotechnol Res Grp, Dept Mol & Appl Biosci, London SW1 6UW, England

[2] ETH, Bioproc Lab, Zurich, Switzerland

[3] Pfizer Ltd, Bioproc Dev Grp, Kalamazoo, MI USA

来源：

BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 05期

关键词：

Oligosaccharide elicitors; Intracellular calcium; Apoaequorin; Secondary metabolites; BACITRACIN-A PRODUCTION; CYTOSOLIC-FREE CA2+; PENICILLIUM-CHRYSOGENUM; RECOMBINANT AEQUORIN; CALCIUM; BACTERIA; CULTURES; LICHENIFORMIS; EFFICIENCY;

D O I：

10.1007/s10529-010-0511-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Elicitation can lead to overproduction of secondary metabolites in plants and microbes. Potential changes in cytosolic Ca2+ levels in bacteria were studied in response to elicitation. We report, for the first time, the effect of oligosaccharide elicitors on intracellular Ca2+ levels. The apoaequorin gene was cloned into Escherichia coli DH5 alpha and Bacillus subtilis 1604 cultures. Addition of elicitors, oligoguluronate and mannan oligosaccharides, to the cultures caused up to 11-fold increase in cytosolic Ca2+ in E. coli and tenfold increase in B. subtilis. These increases in Ca2+ levels could therefore contribute to the enhancement of secondary metabolite levels.

引用

页码：985 / 991

页数：7