Enhanced intracellular Ca2+ concentrations in Escherichia coli and Bacillus subtilis after addition of oligosaccharide elicitors

被引：10
作者：
Murphy, Tania M. [1 ]
Nilsson, Alex Y. [2 ]
Roy, Ipsita [1 ]
Harrop, Anthony [3 ]
Dixon, Keith [3 ]
Keshavarz, Tajalli [1 ]
机构：
[1] Univ Westminster, Appl Biotechnol Res Grp, Dept Mol & Appl Biosci, London SW1 6UW, England
[2] ETH, Bioproc Lab, Zurich, Switzerland
[3] Pfizer Ltd, Bioproc Dev Grp, Kalamazoo, MI USA
来源：
BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 05期
关键词：
Oligosaccharide elicitors; Intracellular calcium; Apoaequorin; Secondary metabolites; BACITRACIN-A PRODUCTION; CYTOSOLIC-FREE CA2+; PENICILLIUM-CHRYSOGENUM; RECOMBINANT AEQUORIN; CALCIUM; BACTERIA; CULTURES; LICHENIFORMIS; EFFICIENCY;
D O I：
10.1007/s10529-010-0511-6
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Elicitation can lead to overproduction of secondary metabolites in plants and microbes. Potential changes in cytosolic Ca2+ levels in bacteria were studied in response to elicitation. We report, for the first time, the effect of oligosaccharide elicitors on intracellular Ca2+ levels. The apoaequorin gene was cloned into Escherichia coli DH5 alpha and Bacillus subtilis 1604 cultures. Addition of elicitors, oligoguluronate and mannan oligosaccharides, to the cultures caused up to 11-fold increase in cytosolic Ca2+ in E. coli and tenfold increase in B. subtilis. These increases in Ca2+ levels could therefore contribute to the enhancement of secondary metabolite levels.
引用
收藏
页码：985 / 991
页数：7
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