Stable isotope-dilution liquid chromatography/tandem mass spectrometry method for determination of thyroxine in saliva

被引：16

作者：

Higashi, Tatsuya ^{[1
,2
]}

Ichikawa, Takuya ^{[1
,2
]}

Shimizu, Chikara ^{[3
]}

Nagai, So ^{[4
]}

Inagaki, Shinsuke ^{[1
,2
]}

Min, Jun Zhe ^{[1
,2
]}

Chiba, Hitoshi ^{[5
]}

Ikegawa, Shigeo ^{[6
]}

Toyo'oka, Toshimasa ^{[1
,2
]}

机构：

[1] Univ Shizuoka, Sch Pharmaceut Sci, Lab Analyt & Bioanalyt Chem, Suruga Ku, Shizuoka 4228526, Japan

[2] Univ Shizuoka, Global COE Program, Suruga Ku, Shizuoka 4228526, Japan

[3] Hokkaido Univ Hosp, Div Lab & Transfus Med, Kita Ku, Sapporo, Hokkaido 0608648, Japan

[4] Hokkaido Univ, Grad Sch Med, Dept Med 2, Kita Ku, Sapporo, Hokkaido 0608638, Japan

[5] Hokkaido Univ, Grad Sch Hlth Sci, Fac Hlth Sci, Kita Ku, Sapporo, Hokkaido 0600812, Japan

[6] Kinki Univ, Fac Pharmaceut Sci, Lab Bioanalyt Sci, Higashiosaka, Osaka 5778502, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2011年 / 879卷 / 13-14期

基金：

日本学术振兴会;

关键词：

Thyroxine; Saliva; Liquid chromatography/electrospray; ionization-tandem mass spectrometry; Isotope dilution; Graves disease; HUMAN SERUM; HORMONE; TOOL;

D O I：

10.1016/j.jchromb.2011.02.048

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) method for the determination of thyroxine (T-4) in human saliva has been developed and validated. The saliva was deproteinized with methanol, purified using a Strata-X (TM) cartridge, and subjected to LC/ESI-MS/MS. Quantification was based on selected reaction monitoring, and [C-13(6)]-T-4 was used as the internal standard. This method allowed the reproducible (intra- and inter-assay relative standard deviations, < 4.8%) and accurate (analytical recovery, 96.5-99.6%) quantification of the salivary T-4 using a 400 mu l sample, and the limit of quantification was 25.0 pg/ml. A preliminary study using the developed method found that there is a diagnosable difference in the salivary T-4 concentration between the euthyroid subjects and the patients with Graves disease. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1013 / 1017

页数：5

共 15 条

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